Anti-5HT4 Receptor抗体(ab60359)
Key features and details
- Rabbit polyclonal to 5HT4 Receptor
- Suitable for: WB, ICC/IF
- Reacts with: Human, Zebrafish
- Isotype: IgG
概述
-
产品名称
Anti-5HT4 Receptor抗体
参阅全部 5HT4 Receptor 一抗 -
描述
兔多克隆抗体to 5HT4 Receptor -
宿主
Rabbit -
特异性
5-HT-4 antibody detects endogenous levels of total 5-HT-4 protein. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human, Zebrafish
预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Opossum
-
免疫原
Synthetic peptide corresponding to Human 5HT4 Receptor aa 21-70.
Sequence:LMAILGNLLV MVAVCWDRQL RKIKTNYFIV SLAFADLLVS
Database link: Q13639 -
阳性对照
- WB: Jurkat cell, COLO205 cells. ICC: HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab60359于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
|
|
| ICC/IF |
1/500 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
|
ICC/IF
1/500 - 1/1000. |
靶标
-
相关性
The 5HT4 Receptor is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulates adenylate cyclase. 5HT4a receptors may play a role in opioid-induced respiratory depression. -
细胞定位
Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli. -
数据库链接
- Entrez Gene: 317708 Cow
- Entrez Gene: 100135548 Guinea pig
- Entrez Gene: 100034053 Horse
- Entrez Gene: 3360 Human
- Entrez Gene: 15562 Mouse
- Entrez Gene: 397431 Pig
- Entrez Gene: 25324 Rat
- Entrez Gene: 710046 Rhesus monkey
see all -
别名
- 5 HT 4 antibody
- 5 HT4 antibody
- 5 HT4R antibody
see all
图片
-
Western blot - Anti-5HT4 Receptor antibody (ab60359)All lanes : Anti-5HT4 Receptor antibody (ab60359)
Lane 1 : Jurkat cell lysate
Lane 2 : COLO205 cell lysate
Lane 3 : COLO205 cell lysate with Synthesized peptide
Predicted band size: 44 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-5HT4 Receptor antibody (ab60359)ab60359, staining 5-HT-4 receptor in HeLa cells by ICC/IF (left), with blocking peptide (right). -
Western blot - Anti-5HT4 Receptor antibody (ab60359)Anti-5HT4 Receptor antibody (ab60359) at 1/1000 dilution + COLO205 cells
Predicted band size: 44 kDa -
Western blot - Anti-5HT4 Receptor antibody (ab60359)All lanes : Anti-5HT4 Receptor antibody (ab60359) at 1/500 dilution
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (12)
ab60359 被引用在 12 文献中.
- She MP et al. Differential roles of serotonin receptor subtypes in regulation of neurotrophin receptor expression and intestinal hypernociception. Histol Histopathol 39:903-919 (2024). PubMed: 38108436
- Wu X et al. Domesticated HERV-W env contributes to the activation of the small conductance Ca2+-activated K+ type 2 channels via decreased 5-HT4 receptor in recent-onset schizophrenia. Virol Sin 38:9-22 (2023). PubMed: 36007838
- Lam SM et al. Quantitative Lipidomics and Spatial MS-Imaging Uncovered Neurological and Systemic Lipid Metabolic Pathways Underlying Troglomorphic Adaptations in Cave-Dwelling Fish. Mol Biol Evol 39:N/A (2022). PubMed: 35277964
- Zhan Y et al. Effects of Maren Pills on the Intestinal Microflora and Short-Chain Fatty Acid Profile in Drug-Induced Slow Transit Constipation Model Rats. Front Pharmacol 13:804723 (2022). PubMed: 35496291
- Chang WY et al. 5-HT7 receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome. Lab Invest 102:1023-1037 (2022). PubMed: 35585132