AB259896

重组Anti-5HT1A Receptor抗体[EPR24117-4]

Anti-5HT1A Receptor antibody [EPR24117-4]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal 5HT1A Receptor antibody. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell line - Rat samples.

查看别名

5ht1a, Htr1a, 5-hydroxytryptamine receptor 1A, 5-HT-1A, 5-HT1A, Serotonin receptor 1A

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-5HT1A Receptor antibody [EPR24117-4] (AB259896)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-5HT1A Receptor antibody [EPR24117-4] (AB259896)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling 5HT1A Receptor with ab259896 at 1/250 (2.316 ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged Htr1a expression vector is observed. ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 4ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-5HT1A Receptor antibody [EPR24117-4] (AB259896)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-5HT1A Receptor antibody [EPR24117-4] (AB259896)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with myc tagged 5HT1A receptor construct cells labelling 5HT1A Receptor with ab259896 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

不同偶联物与剂型 (1)

Carrier free

Anti-5HT1A Receptor antibody [EPR24117-4] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24117-4

亚型

IgG

不含载体蛋白

反应种属

Rat

应用

Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 5HT1A receptor also known as 5-HT1A or serotonin receptor 1A is part of the 5-HT receptor family. This receptor functions mechanically as a G protein-coupled receptor (GPCR) with a molecular mass of approximately 46 kDa. It is widely expressed in the brain with high concentrations in areas like the hippocampus raphe nuclei and cerebral cortex. Additionally you can find 5HT1A receptors in peripheral tissues although at lower levels. They play an essential role in mediating inhibitory neurotransmission by reducing the production of cAMP.

Biological function summary

The 5HT1A receptor is important in regulating mood anxiety and cognition. These receptors function as part of a receptor complex interacting with other serotonin receptors like 5-HT2 and 5-HT3 to modulate neural processes. The 5HT1A receptor mediates its effects by inhibiting adenylate cyclase resulting in decreased levels of intracellular cAMP. This inhibition subsequently affects ion channel function and neurotransmitter release influencing various mental and physiological processes.

Pathways

Several serotonin pathways involve the 5HT1A receptor including the serotonergic and dopaminergic pathways. These pathways are essential for healthy brain function and mood regulation. The receptor also interacts with the protein RGS10 which modulates G protein signaling. RGS10 can influence the pathways the 5HT1A receptor is involved in providing a regulatory mechanism for neurotransmission and receptor activity.

The 5HT1A receptor is linked to depression and anxiety disorders. Abnormalities in the function or expression of this receptor can result in altered serotonergic signaling contributing to these conditions. The receptor's relationship with the protein serotonergic 5-HT2A receptor can modulate the risk and progression of mood disorders. Researchers are actively investigating potential therapies that target the 5-HT1A receptor to alleviate symptoms of these mental health conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 2140514, PubMed : 2156831). Also functions as a receptor for various drugs and psychoactive substances (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (By similarity). HTR1A is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission : signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores (By similarity). Beta-arrestin family members regulate signaling by mediating both receptor desensitization and resensitization processes (By similarity).

See full target information Htr1a

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Abcam Product Promise

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重组Anti-5HT1A Receptor抗体[EPR24117-4]