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AB248255

重组Anti-58K Golgi蛋白抗体[EPR7909] - BSA and Azide free

Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free

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Rabbit Recombinant Monoclonal 58K Golgi protein antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

查看别名

Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, LCHC1, FTCD

4 Images
Western blot - Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free (AB248255)
  • WB

Unknown

Western blot - Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free (AB248255)

This data was developed using ab129005, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-58K Golgi protein antibody [EPR7909] (<a href='/products/primary-antibodies/58k-golgi-protein-antibody-epr7909-ab129005'>ab129005</a>) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free (AB248255)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free (AB248255)

This data was developed using ab129005, the same antibody clone in a different buffer formulation.

ab129005, at 1/250 dilution, staining 58K Golgi protein in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free (AB248255)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free (AB248255)

This data was developed using ab129005, the same antibody clone in a different buffer formulation.

ab129005, at 1/250 dilution, staining 58K Golgi protein in paraffin-embedded Human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free (AB248255)
  • WB

Supplier Data

Western blot - Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free (AB248255)

Blocking buffer and dilution buffer concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-58K Golgi protein antibody [EPR7909] (<a href='/products/primary-antibodies/58k-golgi-protein-antibody-epr7909-ab129005'>ab129005</a>) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

Exposure time: 20s

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7909

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody is not suitable for testing cell line samples in western blot.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 58K Golgi protein also known as Golgi marker protein is a significant component within the Golgi apparatus with a molecular mass of approximately 58 kilodaltons (kDa). This protein is widely expressed in eukaryotic cells and serves as an important marker for studying the structure and function of the Golgi apparatus. The 58K Golgi protein localizes predominantly to the membranes of the Golgi where it plays an integral role in the maintenance of its architecture and function. Researchers often use this protein in Golgi immunofluorescence studies to visualize and track Golgi dynamics and morphology.
Biological function summary

The 58K Golgi protein contributes to the Golgi's role in modifying sorting and packaging proteins and lipids for secretion or delivery to other organelles. It does not function alone but often interacts with other Golgi markers and proteins forming part of a complex that helps regulate intracellular transport processes. These interactions are important for maintaining Golgi function and cellular protein distribution. By acting as a Golgi biomarker the 58K Golgi protein aids in understanding the trafficking and processing pathways essential for cellular function.

Pathways

The 58K Golgi protein is involved in the vesicular transport pathway which is essential for trafficking proteins between the endoplasmic reticulum (ER) and the Golgi apparatus. It interacts with other proteins like COPI and COPII which are critical for vesicle formation and movement through this pathway. This protein also plays a role in the glycosylation pathway where nascent proteins undergo carbohydrate modification in the Golgi. Such modifications assist in protein folding stability and cell signaling. The integration of 58K Golgi protein in these pathways highlights its importance in sustaining cellular operations.

The 58K Golgi protein has correlations with some pathologies associated with Golgi dysfunction including congenital disorders of glycosylation and neurodegenerative diseases such as Alzheimer's disease. Disruptions in Golgi structure and function can lead to improper protein sorting and trafficking which is observed in these disorders. Proteins such as amyloid precursor protein (APP) in Alzheimer's are impacted by disruptions in the Golgi pathway where 58K Golgi protein plays a role. Understanding these connections can help in developing therapeutic strategies targeting Golgi-associated dysfunctions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.. Binds and promotes bundling of vimentin filaments originating from the Golgi.
See full target information FTCD
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