Anti-58K Golgi蛋白抗体[58K-9] - Golgi Marker (ab27043)
![Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)](https://www.abcam.cn/ps/products/27/ab27043/Images/ab27043-542632-anti-58k-golgi-protein-antibody-58k-9-golgi-marker-western-blot-human-liver-hepg2-rat-liver-human-brain.jpg "Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)")
Key features and details
- Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
- Suitable for: ICC, WB, Flow Cyt (Intra)
- Reacts with: Rat, Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-58K Golgi蛋白抗体[58K-9] - Golgi Marker
参阅全部 58K Golgi protein 一抗 -
描述
小鼠单克隆抗体[58K-9] to 58K Golgi蛋白- Golgi Marker -
宿主
Mouse -
经测试应用
适用于: ICC, WB, Flow Cyt (Intra)more details -
种属反应性
与反应: Rat, Human
预测可用于: Hamster, Cow, Dog, Pig, Monkey, African green monkey
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免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Rat Liver and HeLa cells. For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extract Antigen M.W.: 58 kDa
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
58K-9 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27043于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (3) |
Use a concentration of 5 - 10 µg/ml.
Previous batches have worked at the concentration of 1µg/ml. Our current batch appears to work between 5 and 10 µg/ml. Please see the data below for more details. |
| WB | (2) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
|
| Flow Cyt (Intra) |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
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ICC
Use a concentration of 5 - 10 µg/ml. Previous batches have worked at the concentration of 1µg/ml. Our current batch appears to work between 5 and 10 µg/ml. Please see the data below for more details. |
|
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
|
Flow Cyt (Intra)
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi. -
通路
Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion. -
疾病相关
Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. -
序列相似性
In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family. -
细胞定位
Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole. - Information by UniProt
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数据库链接
- Entrez Gene: 10841 Human
- Entrez Gene: 397517 Pig
- Entrez Gene: 89833 Rat
- Omim: 606806 Human
- SwissProt: O95954 Human
- SwissProt: P53603 Pig
- SwissProt: O88618 Rat
- Unigene: 415846 Human
see all -
别名
- Formimidoyltetrahydrofolate cyclodeaminase antibody
- Formimidoyltransferase cyclodeaminase antibody
- Formiminotetrahydrofolate cyclodeaminase antibody
see all
图片
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Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)All lanes : Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) at 2.5 µg/ml
Lane 1 : Human Liver cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Rat Liver cell lysate
Lane 4 : Human Brain cell lysate
Lane 5 : SH-SY5Y cell lysate
Lane 6 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDaWestern blot: Anti-FTCD antibody [58K-9] (ab27043) staining at 2.5 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab27043 was shown to bind specifically to FTCD. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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![Flow Cytometry (Intracellular) - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)](https://www.abcam.cn/ps/products/27/ab27043/Images/ab27043-524189-anti-58K-Golgi-protein-antibody-58K-9-Golgi-Marker-Human-Hep-G2-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)Overlay histogram showing HepG2 cells stained with ab27043 (red line). The cells were PFA-fixed and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27043, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)](https://www.abcam.cn/ps/products/27/ab27043/Images/ab27043-20-anti-58k-golgi-protein-58k9-immunocytochemistry-antibody-hela-human.jpg)
Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)ab27043 staining 58K Golgi protein - Golgi Marker in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab27043 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)](https://www.abcam.cn/ps/products/27/ab27043/Images/ab27043-37831-anti-58k-golgi-protein-antibody-58k-9-golgi-marker-immunofluorescence.jpg)
Immunocytochemistry - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab27043 (1µg/ml, 5µg/ml and 10µg/ml) staining 58K Golgi protein in SK-N-SH cells (green). Cells were fixed in Methanol, permabilised using 0.5% Triton X100 in PBS and counterstained with DAPI in order to highlight the nucleus (red). -
![Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)](https://www.abcam.cn/ps/products/27/ab27043/Images/ab27043-37832-anti-58k-golgi-protein-antibody-58k-9-golgi-marker-western-blot.jpg)
Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)All lanes : Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate, blocked with 5% BSA
Lane 2 : Liver (Rat) Tissue Lysate, blocked with 3% Milk
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Exposure time: 3 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (66)
ab27043 被引用在 66 文献中.
- Scott H et al. The human discs large protein 1 interacts with and maintains connexin 43 at the plasma membrane in keratinocytes. J Cell Sci 136:N/A (2023). PubMed: 37288673
- Zhang X et al. Exostosin glycosyltransferase 1 reduces porcine reproductive and respiratory syndrome virus infection through proteasomal degradation of nsp3 and nsp5. J Biol Chem 298:101548 (2022). PubMed: 34971707
- Patel M et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. Br J Cancer 126:1704-1714 (2022). PubMed: 35173303
- Lee HJ et al. Nalfurafine Hydrochloride, a κ-Opioid Receptor Agonist, Induces Melanophagy via PKA Inhibition in B16F1 Cells. Cells 12:N/A (2022). PubMed: 36611940
- Stefopoulos G et al. Bistability of Dielectrically Anisotropic Nematic Crystals and the Adaptation of Endothelial Collectives to Stress Fields. Adv Sci (Weinh) 9:e2102148 (2022). PubMed: 35344288