Anti-53BP2/ASPP2/BBP抗体(ab70548)
Key features and details
- Rabbit polyclonal to 53BP2/ASPP2/BBP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-53BP2/ASPP2/BBP抗体
参阅全部 53BP2/ASPP2/BBP 一抗 -
描述
兔多克隆抗体to 53BP2/ASPP2/BBP -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human 53BP2/ASPP2/BBP.
Database link: NP_001026855.1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70548于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 125 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 125 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42. -
组织特异性
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines. -
序列相似性
Belongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain. -
结构域
The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53. -
细胞定位
Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42. - Information by UniProt
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数据库链接
- Entrez Gene: 7159 Human
- Omim: 602143 Human
- SwissProt: Q13625 Human
- Unigene: 523968 Human
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别名
- 53BP2 antibody
- Apoptosis stimulating of p53 protein 2 antibody
- Apoptosis stimulating protein of p53 2 antibody
see all
图片
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Western blot - Anti-53BP2/ASPP2/BBP antibody (ab70548)All lanes : Anti-53BP2/ASPP2/BBP antibody (ab70548) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 125 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (ab70548)1mg whole cell lysate from HeLa cells was immunoprecipitated with ab70548 at 3ug/mg of lysate(lane 1) or a control IgG (lane 2). Fr the subsequent blot, 20% of immnunoprecipitate was loaded per lane, and probed with ab70548 at 1ug/ml. Detection: chemiluminescence with exposure time of 30 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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