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AB181377

重组Anti-53BP2/ASPP2/BBP抗体[EPR13837]

Anti-53BP2/ASPP2/BBP antibody [EPR13837]

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(10 Publications)

Rabbit Recombinant Monoclonal 53BP2/ASPP2/BBP antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 10 publications.

查看别名

ASPP2, BBP, TP53BP2, Apoptosis-stimulating of p53 protein 2, Bcl2-binding protein, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53-binding protein 2, Bbp, 53BP2, p53-binding protein 2, p53BP2

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling 53BP2/ASPP2/BBP with ab181377 (unpurified) at a 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling 53BP2/ASPP2/BBP with ab181377 (unpurified) at a 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)
  • WB

Lab

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

The molecular weight observed is consistent with what has been described in PMID : 14766226

All lanes:

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution

All lanes:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 126 kDa

Observed band size: 150 kDa

false

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)
  • WB

Lab

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

The molecular weight observed is consistent with what has been described in PMID : 14766226

All lanes:

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution

Lane 1:

Mouse brain lysates at 20 µg

Lane 2:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 126 kDa

Observed band size: 150 kDa

false

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)
  • WB

Supplier Data

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

All lanes:

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Saos-2 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Predicted band size: 126 kDa

false

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)
  • WB

CiteAb

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (AB181377)

53BP2/ASPP2/BBP western blot using anti-53BP2/ASPP2/BBP antibody [EPR13837] ab181377. Publication image and figure legend from Wu, T., Song, H., et al., 2020, Biomed Res Int, PubMed 32420372.

ab181377 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab181377 please see the product overview.

MiR-30b-5p regulates the expression of ASPP2/AKT axis. (a) MiR-30b-5p suppressed mRNA expression of ASPP2 in MDA-MB-231 and HCC 1937 cells. (b) MiR-30b-5p inhibitor increased mRNA expression of ASPP2. (c) MiR-30b-5p suppressed protein expression of ASPP2 but contributed to the activation of p-AKT in MDA-MB-231 and HCC 1937 cells. (d) MiR-30b-5p inhibitor increased protein expression of ASPP2 and prevented the activation of p-AKT. Data are represented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13837

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p><p>Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p><p>Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p><p>Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

53BP2 also known as ASPP2 or BBP is a protein that interacts with the p53 tumor suppressor. It has a mass of approximately 140 kDa and is expressed in various tissues including the brain liver and lung. Mechanically 53BP2 modulates the activity of p53 influencing cellular apoptosis. The protein consists of several domains such as ankyrin repeats and SH3 domain that facilitate its interaction with multiple partners enhancing its regulatory function.
Biological function summary

53BP2 plays a role in apoptosis and cell cycle control. It acts as a co-activator in the p53 signaling pathway promoting the pro-apoptotic functions of p53. This protein forms part of a complex network involving p53 and other regulatory molecules which ultimately governs important cellular processes like growth arrest and death. By enhancing p53 transcriptional activity 53BP2 aids in maintaining cellular homeostasis and preventing uncontrolled cell proliferation.

Pathways

53BP2 is primarily involved in the p53 signaling and apoptosis pathways. It works closely with key proteins such as p53 and Bcl-2 enhancing the apoptotic response. In the p53 pathway 53BP2 strengthens p53's ability to transactivate genes essential for apoptosis while in the apoptotic pathway it interacts with members of the Bcl-2 family to regulate mitochondrial events leading to cell death.

53BP2's function is relevant in cancer and neurodegenerative diseases. In cancer dysregulation of 53BP2 function or expression can lead to impaired apoptotic response contributing to tumorigenesis. It is linked to proteins like p53 and Bcl-2 in the context of cancer. In neurodegenerative diseases alterations in 53BP2 activity can affect neuronal cell survival with potential connections to proteins involved in synaptic pathways and apoptotic regulation.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53 (PubMed : 12524540). Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of NAE1 to conjugate NEDD8 to CUL1, and thereby decreases NAE1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
See full target information TP53BP2
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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102221 PubMed38868363

2024

LNA-i-miR-221 activity in colorectal cancer: A reverse translational investigation.

Applications

Unspecified application

Species

Unspecified reactive species

Asad Ali,Katia Grillone,Serena Ascrizzi,Giulio Caridà,Lucia Fiorillo,Domenico Ciliberto,Nicoletta Staropoli,Pierosandro Tagliaferri,Pierfrancesco Tassone,Maria Teresa Di Martino

Cell reports 41:111726 PubMed36450254

2022

The ribosomal RNA processing 1B:protein phosphatase 1 holoenzyme reveals non-canonical PP1 interaction motifs.

Applications

Unspecified application

Species

Unspecified reactive species

Gautam Srivastava,Rakhi Bajaj,Ganesan Senthil Kumar,Antoine Gaudreau-Lapierre,Hannah Nicolas,Delphine Chamousset,Dale Kreitler,Wolfgang Peti,Laura Trinkle-Mulcahy,Rebecca Page

Frontiers in immunology 13:974872 PubMed36466835

2022

Dysregulated hepatic lipid metabolism and gut microbiota associated with early-stage NAFLD in ASPP2-deficiency mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Xie,Hang-Fei Xu,Jing Zhang,Xiao-Ni Liu,Bu-Xin Kou,Meng-Yin Cai,Jing Wu,Jin-Ling Dong,Qing-Hua Meng,Yi Wang,Dexi Chen,Yang Zhang

Experimental and therapeutic medicine 24:726 PubMed36340606

2022

ASPP2 promotes cell apoptosis in cervical cancer through inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Feiyun Jiang,Ganxia Bian,Jiehua Li

Biomarker research 9:74 PubMed34635181

2021

Deciphering genes associated with diffuse large B-cell lymphoma with lymphomatous effusions: A mutational accumulation scoring approach.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Abdollahi,Seyedeh Zahra Dehghanian,Liang-Yi Hung,Shiang-Jie Yang,Dao-Peng Chen,L Jeffrey Medeiros,Jung-Hsien Chiang,Kung-Chao Chang

Experimental and therapeutic medicine 21:397 PubMed33680119

2021

expression predicts the prognosis of patients with hepatocellular carcinoma after transcatheter arterial chemoembolization.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaren Mao,Zhongjun Tan,Xiaoqi Pan,Feijian Meng

BioMed research international 2020:7907269 PubMed32420372

2020

Mir-30b-5p Promotes Proliferation, Migration, and Invasion of Breast Cancer Cells via Targeting ASPP2.

Applications

Unspecified application

Species

Unspecified reactive species

Tianqi Wu,Hongming Song,Dan Xie,Kaiyao Hua,Jiashu Hu,Yijun Deng,Changle Ji,Lin Fang

Oncology letters 17:4976-4984 PubMed31186708

2019

TP53BP2 decreases cell proliferation and induces autophagy in neuroblastoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Pang,Lianhong Pan,Yonghui Zhang,Guiyuan Liu

International journal of oncology 52:2001-2010 PubMed29568874

2018

Silencing of ASPP2 promotes the proliferation, migration and invasion of triple-negative breast cancer cells via the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tianqi Wu,Hongming Song,Dan Xie,Bingkun Zhao,Hui Xu,Chenyang Wu,Kaiyao Hua,Yijun Deng,Changle Ji,Jiashu Hu,Lin Fang

The Kaohsiung journal of medical sciences 34:321-329 PubMed29747775

2018

Expression and significance of ASPP2 in squamous carcinoma of esophagus.

Applications

WB, IHC-P

Species

Human, Human

Bo Liu,Lv Yang,Xiu-Juan Li,Rou Li,Wei Sun,Xiao-Yi Chen,Jun-Chao Liu
View all publications
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