Anti-53BP1 (phospho S25)抗体(ab70323)
Key features and details
- Rabbit polyclonal to 53BP1 (phospho S25)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-53BP1 (phospho S25)抗体
参阅全部 53BP1 一抗 -
描述
兔多克隆抗体to 53BP1 (phospho S25) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human 53BP1 (phospho S25).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70323于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately >220 kDa (predicted molecular weight: 214 kDa).
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| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately >220 kDa (predicted molecular weight: 214 kDa). |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
疾病相关
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
序列相似性
Contains 2 BRCT domains. -
翻译后修饰
Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
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数据库链接
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
- Unigene: 481841 Mouse
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别名
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 (phospho S25) antibody (ab70323)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse teratoma (right) tissues labelling 53BP1 (phospho S24) with ab70323 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-53BP1 (phospho S25) antibody (ab70323)All lanes : Anti-53BP1 (phospho S25) antibody (ab70323) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells, untreated
Lane 2 : Whole cell lysate from 293T cells treated
with NCS (200 ng/ml for 1h)
Lysates/proteins at 50 µg per lane.
Predicted band size: 214 kDa
Observed band size: >220 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab70323 被引用在 5 文献中.
- Shi X et al. FLASH X-ray spares intestinal crypts from pyroptosis initiated by cGAS-STING activation upon radioimmunotherapy. Proc Natl Acad Sci U S A 119:e2208506119 (2022). PubMed: 36256824
- Callen E et al. 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination. Mol Cell 77:26-38.e7 (2020). PubMed: 31653568
- Guo H et al. Multi-omics analyses of radiation survivors identify radioprotective microbes and metabolites. Science 370:N/A (2020). PubMed: 33122357
- Lee V et al. Voriconazole enhances UV-induced DNA damage by inhibiting catalase and promoting oxidative stress. Exp Dermatol 29:29-38 (2020). PubMed: 31519066
- Mu F et al. Mangiferin induces radiosensitization in glioblastoma cells by inhibiting nonhomologous end joining. Oncol Rep 40:3663-3673 (2018). PubMed: 30272350