AB124951

重组Anti-5-HT2C Receptor抗体[EPR6486]

Anti-5-HT2C Receptor antibody [EPR6486]

20ul selling size
RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal 5-HT2C Receptor antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

查看别名

HTR1C, HTR2C, 5-hydroxytryptamine receptor 2C, 5-HT-2C, 5-HT2C, 5-HTR2C, 5-hydroxytryptamine receptor 1C, Serotonin receptor 2C, 5-HT-1C, 5-HT1C

2 Images

Unknown

Western blot - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

All lanes:

Western blot - Anti-5-HT2C Receptor antibody [EPR6486] (ab124951) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

false

OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6486

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 5-HT2C receptor also known as 5-hydroxytryptamine receptor 2C is a G-protein coupled receptor in the serotonin receptor family. It has a mass of approximately 51 kDa. This receptor primarily expresses in the central nervous system including regions like the choroid plexus. Researchers note the 5-HT2C receptor's significant presence in areas responsible for regulating mood and cognition making it a critical player in neurotransmitter systems.

Biological function summary

The 5-HT2C receptor plays a role in modulating neurotransmitter release. It is not part of a multi-subunit complex; instead it directly interacts with G proteins to exert its function. Upon activation by serotonin the receptor can influence the release of other neurotransmitters such as dopamine and norepinephrine. This interaction impacts various physiological processes including appetite control mood regulation and anxiety management.

Pathways

One finds the 5-HT2C receptor involved in the serotonin signaling pathway contributing to mood and behavior regulation. The receptor links to the phosphatidylinositol signaling pathway where it engages proteins like phospholipase C. This connection is important for cellular responses to external stimuli. The 5-HT2C receptor also has relationships with other serotonin receptors like 5-HT2B which share similar signaling mechanisms within these pathways.

The 5-HT2C receptor has associations with conditions such as depression and obesity. Its regulatory role in neurotransmitter release influences mood disorders by affecting serotonin levels. Research has shown that the receptor connects with proteins like dopamine transporter which are relevant in the context of psychiatric disorders. In obesity altered receptor activity may affect appetite and energy balance contributing to weight regulation challenges.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 12970106, PubMed : 18703043, PubMed : 19057895, PubMed : 29398112, PubMed : 7895773). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD) (PubMed : 19057895, PubMed : 29398112). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 18703043, PubMed : 29398112). HTR2C is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed : 18703043, PubMed : 29398112). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed : 29398112). Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelanocortin neurons and the release of CRH that then regulates the release of corticosterone (By similarity). Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress (By similarity). Plays a role in insulin sensitivity and glucose homeostasis (By similarity).

See full target information HTR2C

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0287817 PubMed37788276

2023

Antioxioxidant and antiapoptotic effects of Thymosin β4 in Aβ-induced SH-SY5Y cells via the 5-HTR1A/ERK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Hong Zhang,Kai Ling Chin,Shi-Yan Yan,Rahmawati Pare

Gut microbes 14:2127456 PubMed36195972

2022

spp. and their metabolite lactate protect against acute pancreatitis via inhibition of pancreatic and systemic inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Han Li,Jinyan Xie,Xiuliu Guo,Guilian Yang,Bin Cai,Jingtianyi Liu,Mengjia Yue,Yixin Tang,Gan Wang,Shuxian Chen,Jialin Guo,Xuchen Qi,Donghai Wang,Huijun Zheng,Wei Liu,Hong Yu,Chunfeng Wang,Shu Jeffrey Zhu,Feng Guo

Proteomics 22:e2100141 PubMed34932872

2022

Comprehensive kinomic study via a chemical proteomic approach reveals kinome reprogramming in hepatocellular carcinoma tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Wang,Xinzheng Wang,Xin Yang,Feng Liu,Jin Li,Weihua Li,Zhaofang Bai,Hongbo Wang,Jie Mao,Tingting Li,Kun He,Hongxia Wang

View all publications

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重组Anti-5-HT2C Receptor抗体[EPR6486]

Anti-5-HT2C Receptor antibody [EPR6486]

Western blot - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原