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Anti-4 Hydroxynonenal抗体[HNEJ-2] (ab48506)

Reviews (10)Q&A (14)References (158)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)

Key features and details

  • Mouse monoclonal [HNEJ-2] to 4 Hydroxynonenal
  • Suitable for: IHC-P, IHC-FoFr
  • Reacts with: Species independent
  • Isotype: IgG1

概述

  • 产品名称

    Anti-4 Hydroxynonenal抗体[HNEJ-2]
    参阅全部 4 Hydroxynonenal 一抗

  • 描述

    小鼠单克隆抗体[HNEJ-2] to 4 Hydroxynonenal

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, IHC-FoFrmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Chemical/ Small Molecule corresponding to 4 Hydroxynonenal conjugated to keyhole limpet haemocyanin.

  • 阳性对照

    • Colorectal carcinoma cells.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 28th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab48506于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (3)
1/25.
IHC-FoFr (1)
1/25.
说明
IHC-P
1/25.
IHC-FoFr
1/25.

靶标

  • 相关性

    Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.

  • 细胞定位

    Cytoplasmic

  • 别名

    • 4 HNE antibody
    • 4-Hydroxy-2-Nonenal antibody

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)Lu, L. et al PLoS One. 2015 Dec 3;10(12):e0144068. doi: 10.1371/journal.pone.0144068. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Elevated brain oxidative damage in ischemic 5xFAD mice

    Formalin-fixed, paraffin-embedded mouse brain tissue (from non-trasngenic or 5xFAD transgenic animals) stained for 4 Hydroxynonenal using ab48506 at  in immunohistochemical analysis.

    Scale bar = 20μm. (A2) and (B2) are representative images of 4-HNE staining (Red) in brain cortex and CA1 region, respectively.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)This image was kindly supplied by Dr Jinqing Li by Abreview
    ab48506 at a 1/25 dilution staining 4-Hydroxy-2-Nonenal in mouse heart tissue sections by Immunohistochemistry (paraffin embedded) incubated for 15 hours at +4°C. Fixed with formaldehyde, heat mediated antigen retrieval step performed using citrate buffer. Blocked using 5% serum for 20 minutes at 20°C. Secondary used undiluted polyclonal Goat anti-mouse IgG conjugated to Alexa Fluor 594.

    See Abreview

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)This image is courtesy of an Abreview submitted by Seontae Kim
    ab15463 staining 4-Hydroxynonenal in the Mouse melanoma cancer tissue sections by IHC-FoFr (PFA perfusion fixed frozen Sections). Tissue samples were fixed with 4% Formalin, Antigen retrieval carried out heat mediation using anautoclave for 10 minutes, permeabilized using 0.1% SDS and blocked with 1% BSAfor 15 minutes at 25°C. The sample was incubated with primary antibody (1/25 in PBS + 1% BSA) at 4°C for 12 hours.An Alexa Fluor®488-conjugated Goat anti-mouse IgG(H+L) monoclonal(1/100) was used as the secondary antibody.

    See Abreview

文献 (158)

发表研究结果有使用 ab48506？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab48506 被引用在 158 文献中.

  • Xu X  et al. Increased ATF2 expression predicts poor prognosis and inhibits sorafenib-induced ferroptosis in gastric cancer. Redox Biol 59:102564 (2023). PubMed: 36473315
  • Jo J  et al. Protective Effects of Orexin A in a Murine Model of Cisplatin-Induced Acute Kidney Injury. J Clin Med 11:N/A (2022). PubMed: 36498769
  • Guo J  et al. Hepatocyte-specific TMEM16A deficiency alleviates hepatic ischemia/reperfusion injury via suppressing GPX4-mediated ferroptosis. Cell Death Dis 13:1072 (2022). PubMed: 36572666
  • Cao L  et al. Traditional Chinese medicine Lingguizhugan decoction ameliorate HFD-induced hepatic-lipid deposition in mice by inhibiting STING-mediated inflammation in macrophages. Chin Med 17:7 (2022). PubMed: 34983596
  • Zhang X  et al. Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma. Cancer Commun (Lond) 42:287-313 (2022). PubMed: 35184419
View all Publications for this product

客户评价及客户问答

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1-10 of 24 Abreviews or Q&A

Immunocytochemistry abreview for Anti-4 Hydroxynonenal antibody [HNEJ-2]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (ipsc-derived human midbrain dopaminergic neurons)
Permeabilization
No
Specification
ipsc-derived human midbrain dopaminergic neurons
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Idan Rosh

Verified customer

提交于 Sep 02 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-4 Hydroxynonenal antibody [HNEJ-2]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Kidney cells)
Permeabilization
Yes - 0.1% saponin
Specification
Kidney cells
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Hana Valenta

Verified customer

提交于 Aug 05 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-4 Hydroxynonenal antibody [HNEJ-2]

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate, 0.5% Tween 20, pH 6.0
Permeabilization
Yes - PBS/0.2% Triton-X-100
Specification
retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 05 2020

Western blot abreview for Anti-4 Hydroxynonenal antibody [HNEJ-2]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (8%)
Loading amount
35 µg
Treatment
5% ethanol for 10 days
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Ji Hye Han

Verified customer

提交于 Oct 23 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-4 Hydroxynonenal antibody [HNEJ-2]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
None
Permeabilization
No
Specification
Liver
Blocking step
Vector Lab M.O.M. blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.8% · Temperature: 25°C
Fixative
Bouin's fixative
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 19 2016

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-4 Hydroxynonenal antibody [HNEJ-2]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Melanoma cancer)
Specification
Melanoma cancer
Fixative
4% Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: autoclaved for 10min
Permeabilization
Yes - 0.1% SDS
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Seontae Kim

Verified customer

提交于 Dec 19 2012

Western blot abreview for Anti-4 Hydroxynonenal antibody [HNEJ-2]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (B16F10 melanoma)
Loading amount
30 µg
Specification
B16F10 melanoma
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Seontae Kim

Verified customer

提交于 Dec 18 2012

Question

Will this antibody detect free 4-HNE as well, or only 4-HNE modified proteins?

Read More
Answer

ab48506 antibody will detect only 4-HNE modified proteins.

This antibody is specific for 4-HNE-lysine, 4-HNE-histidine and
4-HNE-cysteine adduct.
By inhibition test, this antibody has a much higher affinity for the
4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE cysteine adduct.

ab48506 shows almost negligible reactivity with proteins that were
treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal,
4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde.

Read More

Question

Hola
El anticuerpo ab48506 está garantizado para tejidos de ratón? Nuestro cliente quiere probar con secciones de cerebro.
Muchas Gracias. Saludos.

Read More
Answer

Gracias por contactarnos.


No especificamos las especies para las que este anticuerpo está recomendado pues el antígeno corresponde a una estructura química, una molécula relativamente pequeña que es constante en todas las especies.

Siempre y cuando se exprese en el tejido que el cliente quiere usar, no habría problema en probarlo con secciones de ratón.

Tened en cuenta que en nuestro laboratorio se ha validado en secciones embebidas en parafina y IHC-FoFr.

Si tenéis cualquier otra consulta, no dudes en contactarnos de nuevo.

Read More

Question

Dear Sir,
I purchased Mouse monoclonal [HNEJ-2] to 4 Hydroxynonenal antibody from abcam. I plan to use it for IHC-P. What solution or buffer do you recommend for diluting it prior to use? Can I use Dako products?
And can i use DACO Envision system for visualisation?
Thank you in advance for answer!

Read More
Answer

Thank you for contacting us.

For IHC-P we recommend to dilute the anti-4 Hydroxynonenal antibody [HNEJ-2] ab48506 in TBS with 1% BSA at a concentration of 10 - 20 µg/ml.
As indicated on the product datasheet, a heat mediated antigen retrieval via the microwave method can be performed before commencing with IHC staining protocol. But a fixation of the samples using Bouin's solution is recommended. In this case, antigen retrieval process is not required.

The use of Dako products should be fine, however we have no such evidence for this particular antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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1-10 of 24 Abreviews or Q&A

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