AB247373

重组Anti-4-1BBL抗体[EPR1172Y] - BSA and Azide free

Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal 4-1BBL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

查看别名

Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9

4 Images

Lab

Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)

This data was developed using ab68185, the same antibody clone in a different buffer formulation.

ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.

Lane 1 (input) : HEK293 whole cell lysate (10μg)

Lane 2 (+) : ab68185 + HEK293 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (<a href='/products/primary-antibodies/4-1bbl-antibody-epr1172y-ab68185'>ab68185</a>)

Predicted band size: 27 kDa

Observed band size: 25 kDa,32 kDa

false

Lab

Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)

This data was developed using ab68185, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-4-1BBL antibody [EPR1172Y] (<a href='/products/primary-antibodies/4-1bbl-antibody-epr1172y-ab68185'>ab68185</a>) at 1/1000 dilution

All lanes:

HEK293 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 27 kDa

Observed band size: 25-32 kDa

false

Unknown

Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)

This data was developed using ab68185, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-4-1BBL antibody [EPR1172Y] (<a href='/products/primary-antibodies/4-1bbl-antibody-epr1172y-ab68185'>ab68185</a>) at 1/2000 dilution

All lanes:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 27 kDa

Observed band size: 25 kDa

false

Lab

Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)

This data was developed using ab68185, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-4-1BBL antibody [EPR1172Y] (<a href='/products/primary-antibodies/4-1bbl-antibody-epr1172y-ab68185'>ab68185</a>) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 27 kDa

Observed band size: 25 kDa,32 kDa

false

不同偶联物与剂型 (2)

Unconjugated

Anti-4-1BBL antibody [EPR1172Y]
HRP

HRP Anti-4-1BBL antibody [EPR1172Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR1172Y

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

ab247373 is the carrier-free version of ab68185.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 4-1BBL protein also known as CD137L or 4-1BB ligand acts mechanically as a member of the tumor necrosis factor (TNF) superfamily. The 4-1BBL with a mass of around 36 kDa exists as a type II transmembrane protein. It gets expressed on activated antigen-presenting cells like dendritic cells macrophages and B cells. This costimulatory molecule primarily interacts with its receptor 4-1BB (CD137) on T cells promoting their activation.

Biological function summary

The 4-1BBL protein plays an important role in mediating the co-stimulatory signal needed for T-cell proliferation survival and cytokine production. This protein contributes to the formation of the immunological synapse and doesn't exist alone but rather interacts directly with its receptor CD137 forming a receptor-ligand complex. The 4-1BBL-CD137 interaction strengthens signals delivered by the T-cell receptor enhancing immune responses.

Pathways

The 4-1BBL protein integrates into immune-related signaling pathways. It significantly impacts the NF-kB and MAPK pathways important for T cell survival and activation. The interaction between 4-1BBL and its related proteins such as the 4-1BB/CD137 receptor initiates downstream cascades that lead to the activation of transcription factors involved in immune response.

The 4-1BBL protein connects to conditions such as cancer and autoimmune diseases. Overexpression or dysregulation of 4-1BBL has associations with autoimmune conditions like rheumatoid arthritis due to prolonged T cell activation. In cancer 4-1BBL's interaction with CD137 has potential therapeutic implications with efforts to harness this axis to bolster anti-tumor immunity. The modulation of 4-1BBL activity can influence disease outcomes highlighting its significance as a target for therapeutic interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.

See full target information TNFSF9

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重组Anti-4-1BBL抗体[EPR1172Y] - BSA and Azide free