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Anti-2B4抗体(ab113289)

Reviews (3)Q&A (1)References (1)
Western blot - Anti-2B4 antibody (ab113289)
  • Immunocytochemistry/ Immunofluorescence - Anti-2B4 antibody (ab113289)

Key features and details

  • Rabbit polyclonal to 2B4
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-2B4抗体
    参阅全部 2B4 一抗

  • 描述

    兔多克隆抗体to 2B4

  • 宿主

    Rabbit

  • 特异性

    At least three isoforms are known to exist. ab113289 is predicted to not cross-react with other family members.

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human 2B4 (C terminal).

  • 阳性对照

    • Daudi cell lysate.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab113289于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF
Use a concentration of 20 µg/ml.
说明
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF
Use a concentration of 20 µg/ml.

靶标

  • 功能

    Modulate other receptor-ligand interactions to enhance leukocyte activation.

  • 组织特异性

    Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils.

  • 序列相似性

    Contains 2 Ig-like (immunoglobulin-like) domains.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 2B4 antibody
      • C9.1 antibody
      • CD244 antibody
      • CD244 antigen antibody
      • CD244 molecule antibody
      • CD244 molecule natural killer cell receptor 2B4 antibody
      • CD244 natural killer cell receptor 2B4 antibody
      • CD244_HUMAN antibody
      • F730046O15Rik antibody
      • h2B4 antibody
      • Ly90 antibody
      • NAIL antibody
      • Natural killer cell activation-inducing ligand antibody
      • Natural killer cell receptor 2B4 antibody
      • NK cell activation inducing ligand antibody
      • NK cell activation inducing ligand NAIL antibody
      • NK cell activation-inducing ligand antibody
      • NK cell type I receptor protein 2B4 antibody
      • NKR2B4 antibody
      • Nmrk antibody
      • Non-MHC restricted killing associated antibody
      • OTTHUMP00000027884 antibody
      • p38 antibody
      • signaling lymphocytic activation molecule 4 antibody
      • SLAM family, member 4 antibody
      • SLAMF4 antibody
      see all

    图片

    • Western blot - Anti-2B4 antibody (ab113289)
      Western blot - Anti-2B4 antibody (ab113289)
      Lane 1 : Anti-2B4 antibody (ab113289) at 1 µg/ml
      Lane 2 : Anti-2B4 antibody (ab113289) at 2 µg/ml

      All lanes : Daudi cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 42 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-2B4 antibody (ab113289)
      Immunocytochemistry/ Immunofluorescence - Anti-2B4 antibody (ab113289)

      Immunofluorescence analysis of Daudi cells, staining 2B4 with ab113289 at 20 µg/ml.

    文献 (1)

    发表研究结果有使用 ab113289？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab113289 被引用在 1 文献中.

    • Feng S  et al. Proton pump inhibitor pantoprazole inhibits the proliferation, self-renewal and chemoresistance of gastric cancer stem cells via the EMT/ß-catenin pathways. Oncol Rep 36:3207-3214 (2016). PubMed: 27748935

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-2B4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cat Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH 6.0
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 27 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-2B4 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 01 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-2B4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (spleen)
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 01 2016

    Question

    One remaining question, though. The western blot figure shows a band at ˜30kD, but the figure legend states the predicted molecular weight as 42. Can you explain this discrepancy? https://www.abcam.com/CD244-antibody-ab113289.html#description_images_1

    Read More
    Answer


    According to the SwissProt link below, CD244 has 4 isoforms that are 42, 41, 37, and 31 kDa. Thus, it appears the band in the image you referenced likely corresponds to the 31 kDa isoform 4.
    http://www.uniprot.org/uniprot/Q9BZW8

    Read More

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