重组Anti-160 kD Neurofilament Medium (phospho S614 + S619)抗体[EPR580(2)Y] (ab68142)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)](https://www.abcam.cn/ps/products/68/ab68142/Images/ab68142-82350-anti-160-kd-neurofilament-medium-phospho-s614-s619-antibody-epr5802y-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-160 kD Neurofilament Medium (phospho S614 + S619)抗体[EPR580(2)Y]
参阅全部 160 kD Neurofilament Medium 一抗 -
描述
兔单克隆抗体[EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- SH-SY5Y cell lysates; human brain tissue
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR580(2)Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68142于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IHC-P |
| 说明 |
|---|
应用说明
IHC-P: 1/100 - 1/250.
WB: 1/500. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa).
Is unsuitable for Flow Cyt, ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa).
Is unsuitable for Flow Cyt, ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. - Information by UniProt
-
数据库链接
- Entrez Gene: 4741 Human
- Omim: 162250 Human
- SwissProt: P07197 Human
- Unigene: 458657 Human
- Unigene: 730072 Human
-
别名
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- NEF3 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68142 at a dilution of 1/100 - 1/250
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Western blot - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)](https://www.abcam.cn/ps/products/68/ab68142/Images/ab68142-82349-anti-160-kd-neurofilament-medium-phospho-s614-s619-antibody-epr5802y-western-blot.jpg)
Western blot - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)All lanes : Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142) at 1/500 dilution
Lane 1 : SH-SY5Y cell lysate treated with Alkaline phosphatase
Lane 2 : Untreated SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted? -
![Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)](https://www.abcam.cn/ps/products/68/ab68142/Images/ab68142-3-benefits-of-recombinant-antibodies.png)
Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab68142 尚未被引用在任何文献中。