重组Anti-160 kD Neurofilament Medium抗体[EPR23950-2] - BSA and Azide free (Capture) (ab281199)
![Anti-160 kD Neurofilament Medium antibody [EPR23950-2] - BSA and Azide free (Capture) (ab281199)](https://www.abcam.cn/ps/products/281/ab281199/Images/ab281199-1-RecombinantAntibodyBenefitfigure.png "Anti-160 kD Neurofilament Medium antibody [EPR23950-2] - BSA and Azide free (Capture) (ab281199)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23950-2] to 160 kD Neurofilament Medium - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
概述
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产品名称
Anti-160 kD Neurofilament Medium抗体[EPR23950-2] - BSA and Azide free (Capture)
参阅全部 160 kD Neurofilament Medium 一抗 -
描述
兔单克隆抗体[EPR23950-2] to 160 kD Neurofilament Medium - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab281199 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human NEFM. The recommended pair for sandwich ELISA is:
Capture: ab281199, Human NEFM Capture Antibody (unconjugated)
Detector: ab281049, Human NEFM Detector Antibody (unconjugated)
The reference range value is 0.16 - 10 ng/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23950-2 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
- Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
- Anti-160 kD Neurofilament Medium antibody [EPR23951-39] - BSA and Azide free (Detector) (ab281049)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281199于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23951-39] to 160 kD Neurofilament Medium - BSA and Azide free (Detector) (ab281049).
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23951-39] to 160 kD Neurofilament Medium - BSA and Azide free (Detector) (ab281049). |
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 4741 Human
- Omim: 162250 Human
- SwissProt: P07197 Human
- Unigene: 458657 Human
- Unigene: 730072 Human
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别名
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- NEF3 antibody
see all
图片
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Anti-160 kD Neurofilament Medium antibody [EPR23950-2] - BSA and Azide free (Capture) (ab281199)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281199 尚未被引用在任何文献中。
