Anti-160 kD Neurofilament Medium抗体(ab134458)
Key features and details
- Chicken polyclonal to 160 kD Neurofilament Medium
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgY
概述
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产品名称
Anti-160 kD Neurofilament Medium抗体
参阅全部 160 kD Neurofilament Medium 一抗 -
描述
鸡多克隆抗体to 160 kD Neurofilament Medium -
宿主
Chicken -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant NFM protein expressed in bacteria followed by hens co-boosted with recombinant NFM protein expressed in bacteria
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常规说明
Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
纯度
IgY fraction -
纯化说明
Immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in buffer and then filter-sterilized. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134458于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/2000.
|
| 说明 |
|---|
|
ICC/IF
1/1000 - 1/2000. |
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 18040 Mouse
- SwissProt: P08553 Mouse
- Unigene: 242832 Mouse
- Unigene: 390700 Mouse
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别名
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- NEF3 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (ab134458)Picture courtesy of Dr. Gerry Shaw, University of Florida
Immunofluorescent analysis of dissociated culture of mouse cortical neurons labelling 160 kD Neurofilament Medium with ab134458 at 1/2000 dilution (green staining). Neurites are stained in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence at 1/5000 dilution. Blue nuclei demonstrate DAPI DNA staining.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab134458 被引用在 3 文献中.
- Wilkerson BA et al. Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris. Elife 10:N/A (2021). PubMed: 34003106
- Lian JJ et al. Flumazenil-Insensitive Benzodiazepine Effects in Recombinant aß and Neuronal GABAA Receptors. Brain Sci 10:N/A (2020). PubMed: 32150806
- DiCello JJ et al. Mu and Delta Opioid Receptors Are Coexpressed and Functionally Interact in the Enteric Nervous System of the Mouse Colon. Cell Mol Gastroenterol Hepatol 9:465-483 (2020). PubMed: 31759144
