Anti-14-3-3 sigma/SFN抗体(ab77187)
Key features and details
- Goat polyclonal to 14-3-3 sigma/SFN
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-14-3-3 sigma/SFN抗体
参阅全部 14-3-3 sigma/SFN 一抗 -
描述
山羊多克隆抗体to 14-3-3 sigma/SFN -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Sheep, Cow, Dog, Pig, Zebrafish, Dictyostelium discoideum, Rainbow trout
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免疫原
Synthetic peptide corresponding to Human 14-3-3 sigma/SFN (internal sequence).
Sequence:DLHTLSEDSYKDST
Database link: P31947 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77187于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 2 - 4 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. |
靶标
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功能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression. -
组织特异性
Present mainly in tissues enriched in stratified squamous keratinizing epithelium. -
序列相似性
Belongs to the 14-3-3 family. -
翻译后修饰
Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. -
细胞定位
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 528453 Cow
- Entrez Gene: 487351 Dog
- Entrez Gene: 2810 Human
- Entrez Gene: 55948 Mouse
- Entrez Gene: 733625 Pig
- Entrez Gene: 313017 Rat
- Entrez Gene: 443032 Sheep
- Omim: 601290 Human
see all -
别名
- 14 3 3 protein sigma antibody
- 14-3-3 protein sigma antibody
- 1433S_HUMAN antibody
see all
图片
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml + Human skin tissue lysate in RIPA buffer at 35 µg
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (ab77187)Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma/SFN with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)All lanes : Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml
Lane 1 : A431 cell lysate in RIPA buffer
Lane 2 : U2OS cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)All lanes : Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml
Lane 1 : Mouse skin tissue lysate in RIPA buffer
Lane 2 : Rat skin tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab77187 被引用在 3 文献中.
- Mayr CH et al. Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers. EMBO Mol Med 13:e12871 (2021). PubMed: 33650774
- Kobayashi Y et al. Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. Nat Cell Biol 22:934-946 (2020). PubMed: 32661339
- Plak K et al. Rap1-dependent pathways coordinate cytokinesis in Dictyostelium. Mol Biol Cell 25:4195-204 (2014). ICC/IF ; Dictyostelium discoideum . PubMed: 25298405
