重组Anti-14-3-3抗体[EPR6380] (ab125032)
![Western blot - Anti-14-3-3 antibody [EPR6380] (ab125032)](https://www.abcam.cn/ps/products/125/ab125032/Images/ab125032-139175-anti-14-3-3-antibody-epr6380-western-blot.jpg "Western blot - Anti-14-3-3 antibody [EPR6380] (ab125032)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6380] to 14-3-3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-14-3-3抗体[EPR6380]
参阅全部 14-3-3 一抗 -
描述
兔单克隆抗体[EPR6380] to 14-3-3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human 14-3-3 aa 200-300. The exact sequence is proprietary.
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阳性对照
- HeLa, SH-SY5Y, Raji and HepG2 whole cell lysate (ab7900), WB: 14-3-3 beta/gamma/epsilon/zeta/eta/theta/sigma Human Recombinant Protein
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6380 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125032于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. -
序列相似性
Belongs to the 14-3-3 family. -
翻译后修饰
The alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Isoform Short contains a N-acetylmethionine at position 1. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 7529 Human
- Entrez Gene: 7534 Human
- Entrez Gene: 22631 Mouse
- Entrez Gene: 54401 Mouse
- Entrez Gene: 25578 Rat
- Entrez Gene: 56011 Rat
- Omim: 601288 Human
- Omim: 601289 Human
see all -
别名
- 14 3 3 antibody
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
see all
图片
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Western blot - Anti-14-3-3 antibody [EPR6380] (ab125032)All lanes : Anti-14-3-3 antibody [EPR6380] (ab125032) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-14-3-3 antibody [EPR6380] (ab125032)](https://www.abcam.cn/ps/products/125/ab125032/Images/ab125032-506364-anti-14-3-3-antibody-epr6380-western-blot-hu-recombinant-protein.png)
Western blot - Anti-14-3-3 antibody [EPR6380] (ab125032)All lanes : Anti-14-3-3 antibody [EPR6380] (ab125032) at 1/1000 dilution
Lane 1 : 14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 2 : 14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 3 : 14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 4 : 14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 5 : 14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 6 : 14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 7 : 14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 60 secondsBlocking and diluting buffer: 5% NFDM/TBST
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![Anti-14-3-3 antibody [EPR6380] (ab125032)](https://www.abcam.cn/ps/products/125/ab125032/Images/ab125032-2-benefits-of-recombinant-antibodies.png)
Anti-14-3-3 antibody [EPR6380] (ab125032)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab125032 被引用在 1 文献中.
- Cocozza F et al. Extracellular vesicles and co-isolated endogenous retroviruses from murine cancer cells differentially affect dendritic cells. EMBO J 42:e113590 (2023). PubMed: 38073509