重组Anti-14-3-3 alpha + beta抗体[Y62] (ab32560)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y62] to 14-3-3 alpha + beta
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-14-3-3 alpha + beta抗体[Y62]
参阅全部 14-3-3 alpha + beta 一抗 -
描述
兔单克隆抗体[Y62] to 14-3-3 alpha + beta -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human 14-3-3 alpha + beta aa 200-300 (C terminal). The exact sequence is proprietary.
-
阳性对照
- WB: Hela cell lysate. IHC-P: Human breast tissue slides. ICC/IF: HeLa cells
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y62 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32560于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100.
|
说明 |
---|
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
靶标
-
功能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. -
序列相似性
Belongs to the 14-3-3 family. -
翻译后修饰
The alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
数据库链接
- Entrez Gene: 7529 Human
- Entrez Gene: 54401 Mouse
- Entrez Gene: 56011 Rat
- Omim: 601289 Human
- SwissProt: P31946 Human
- SwissProt: Q9CQV8 Mouse
- SwissProt: P35213 Rat
- Unigene: 643544 Human
see all -
别名
- 14 3 3 alpha antibody
- 14 3 3 protein beta/alpha antibody
- 14-3-3 protein beta/alpha antibody
see all
图片
-
Anti-14-3-3 alpha + beta antibody [Y62] (ab32560) at 1/1000 dilution + Hela cell lysate.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling 14-3-3 alpha + beta with purified ab32560 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)
Ab32560, at a 1/500 dilution, staining 14-3-3 alpha + beta in paraffin embedded human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab32560 被引用在 3 文献中.
- Lou Y et al. Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis. Cell Death Dis 12:170 (2021). PubMed: 33568630
- Hu X et al. Identification and Validation of Novel Biomarkers for Diagnosis and Prognosis of Hepatocellular Carcinoma. Front Oncol 10:541479 (2020). PubMed: 33102213
- Gao K et al. Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils. J Cell Sci 128:992-1000 (2015). WB, IP ; Human . PubMed: 25588844