重组Anti- IKK beta抗体[EPR16628] (ab178870)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16628] to IKK beta
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti- IKK beta抗体[EPR16628]
参阅全部 IKK beta 一抗 -
描述
兔单克隆抗体[EPR16628] to IKK beta -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, Daudi, HL-60, A431, 293T, C6, Raw264.7 and NIH/3T3 whole cell lysates. Human fetal kidney lysates. Mouse brain, Mouse kidney, Mouse spleen and Rat kidney lysates. IP: Jurkat whole cell extract.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16628 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178870于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/40.
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WB | (1) |
1/1000. Detects a band of approximately 75, 87 kDa (predicted molecular weight: 85, 87 kDa).
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说明 |
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IP
1/40. |
WB
1/1000. Detects a band of approximately 75, 87 kDa (predicted molecular weight: 85, 87 kDa). |
靶标
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功能
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. -
组织特异性
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ. -
细胞定位
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 3551 Human
- Entrez Gene: 16150 Mouse
- Entrez Gene: 84351 Rat
- Omim: 603258 Human
- SwissProt: O14920 Human
- SwissProt: O88351 Mouse
- SwissProt: Q9QY78 Rat
- Unigene: 597664 Human
see all -
别名
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
see all
图片
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All lanes : Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IKKa/b knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 85, 87 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Western blot: Anti-IKKa/b antibody [EPR16628] (ab178870) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab178870 was shown to bind specifically to IKKa/b. A band was observed at 85 kDa in wild-type HeLa cell lysates with no signal observed at this size in IKKa/b knockout cell line. To generate this image, wild-type and IKKa/b knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysates
Lane 3 : HL-60 (Human promyelocytic leukemia cells) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 85, 87 kDa
Observed band size: 75,87 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
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All lanes : Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysates
Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 85, 87 kDa
Observed band size: 75,87 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha. -
Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution + Human fetal kidney lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 85, 87 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
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All lanes : Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Mouse spleen lysates
Lane 4 : Rat kidney lysates
Lane 5 : C6 (Rat glial tumor cells) whole cell lysates
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 85, 87 kDa
Observed band size: 75,87 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha. -
Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
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Cross-Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab32041 (IKK alpha) at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.
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All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysates
Lane 3 : HL-60 (Human promyelocytic leukemia cells) whole cell lysates
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 85, 87 kDa
Observed band size: 75,87 kDa why is the actual band size different from the predicted?This western blot image is a comparison of ab178870 against a competitor's leading rabbit polyclonal antibody.
Blocking/dilution buffer: 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
-
All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200
Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysates
Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 85, 87 kDa
Observed band size: 75,87 kDa why is the actual band size different from the predicted?This western blot image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.
Blocking/dilution buffer: 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
-
Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution + Human fetal kidney lysates
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 85, 87 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?This western blot image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.
Blocking/dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Mouse spleen lysates
Lane 4 : Rat kidney lysates
Lane 5 : C6 (Rat glial tumor cells) whole cell lysates
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 85, 87 kDa
Observed band size: 75,87 kDa why is the actual band size different from the predicted?This western blot image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.
Blocking/dilution buffer: 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
-
This immunoprecipation image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.
Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.
Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using competitor’s Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/12 dilution. Western blot detection was performed using competitor antibody at 1/100 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/100 dilution. The blocking and diluting buffer was 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (57)
ab178870 被引用在 57 文献中.
- Li T et al. The Effect of Bergenin on Isonicotinic Acid Hydrazide and Rifampicin-Induced Liver Injury Revealed by RNA Sequencing. Molecules 28:N/A (2023). PubMed: 37513369
- Su X et al. Astragaloside IV attenuates high glucose-induced NF-κB-mediated inflammation through activation of PI3K/AKT-ERK-dependent Nrf2/ARE signaling pathway in glomerular mesangial cells. Phytother Res 37:4133-4148 (2023). PubMed: 37189016
- Zhang K et al. Targeting GPR65 alleviates hepatic inflammation and fibrosis by suppressing the JNK and NF-κB pathways. Mil Med Res 10:56 (2023). PubMed: 38001521
- Ling X et al. Irf7 regulates the expression of Srg3 and ferroptosis axis aggravated sepsis-induced acute lung injury. Cell Mol Biol Lett 28:91 (2023). PubMed: 37946128
- Khilazheva ED et al. Impact of NLRP3 Depletion on Aging-Related Metaflammation, Cognitive Function, and Social Behavior in Mice. Int J Mol Sci 24:N/A (2023). PubMed: 38068904