MitoBiogenesis™ Western Blot Cocktail (ab123545)

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To our knowledge, ab123545 Mitobiogenesis Western Blot Cocktail has not been tested in Drosophila. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

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Antibodies against to two different proteins are included to allow comparison of relative expression of each protein in treated samples to the relative expression in untreated samples, to identify inhibition of mitobiogenesis. A brief explanation of the rationale can be found in the introductory material in the MitoSciences (now part of Abcam) brochure at the following link:

http://mitosciences.com/PDF/biogenesis_playbook.pdf

"Measuring RNA levels using reverse transcription qPCR is occasionally discussed as a useful high-throughput technique for identifying effects on mitochondrial biogenesis, but this approach has a fundamental limitation, that being its inability to identify inhibition of mitochondrial ribosomes. If such an effect is occuring then RNA levels might in fact increase in the cell, since mitochondrial translation is unable to process transcription products. The only way to easily and simultaneously identify inhibition of both transcription and translation is to ratio the levels of an mtDNA-encoded protein with total cell protein and/or a nDNA-encoded protein."

The Abcam datasheets for the InCell ELISA kits mentioned in that brochure are at the following links:

https://www.abcam.com/MitoBiogenesis™-In-Cell-ELISA-Kit-Colorimetric-ab110217.html

https://www.abcam.com/MitoBiogenesis™-In-Cell-ELISA-Kit-IR-ab110216.html

I hope this information is helpful to you. Please do not hesitate to contact us if you have more questions.

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You may certainly use ab110416 (MS604) MitoProfile® Total OXPHOS Rodent WB Antibody Cocktailto track mitochondrial biogenesis since the cocktail contains COX I which is mtDNA encoded and four antibodies which are nDNA encoded. COX I runs to 40kDa which is the third band. Since this product contains five antibodies which target on complex I, II, III, IV and complex V, it does make things more complicated.

We would like to mention that we do have a mitobiogenesis cocktail (ab123545) which has only three antibodies specifically to monitor mitochondrial biogenesis, COX I (mtDNA encoded), SDHA (nDNA encoded) and Actin beta antibody as a loading control.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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