人Gelsolin抗体Pair - BSA and Azide free (ab253312)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
概述
-
产品名称
人Gelsolin抗体Pair - BSA and Azide free
参阅全部 Gelsolin 试剂盒 -
检测类型
ELISA set -
范围
546.875 pg/ml - 35000 pg/ml -
种属反应性
与反应: Human -
产品概述
The Antibody Pair can be used to quantify Human Gelsolin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab270215), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
-
经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
无载体
是 -
组件 10 x 96 tests Human Gelsolin Capture Antibody (unconjugated) 1 x 100µg Human Gelsolin Detector Antibody (unconjugated) 1 x 100µg -
研究领域
-
功能
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. -
组织特异性
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. -
疾病相关
Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure. -
序列相似性
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats. -
翻译后修饰
Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. -
细胞定位
Cytoplasm > cytoskeleton and Secreted. - Information by UniProt
-
别名
- Actin depolymerizing factor
- Actin-depolymerizing factor
- ADF
see all -
数据库链接
- Entrez Gene: 2934 Human
- Omim: 137350 Human
- SwissProt: P06396 Human
- Unigene: 522373 Human
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab253312于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
Sandwich ELISA - Human Gelsolin Antibody Pair - BSA and Azide free (ab253312)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab270215), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
-
Sandwich ELISA - Human Gelsolin Antibody Pair - BSA and Azide free (ab253312)To learn more about the advantages of recombinant antibodies see here.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab253312 尚未被引用在任何文献中。
