AB253721

人Factor IX (F9)抗体Pair - BSA and Azide free

Human Factor IX (F9) Antibody Pair - BSA and Azide free

RabMAb
Recombinant
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Human Factor IX (F9) Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Factor IX (F9).

查看别名

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9

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Sandwich ELISA - Human Factor IX (F9) Antibody Pair - BSA and Azide free (AB253721)

sELISA

Supplier Data

Sandwich ELISA - Human Factor IX (F9) Antibody Pair - BSA and Azide free (AB253721)

Example of Human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Factor IX (F9) Antibody Pair - BSA and Azide free (AB253721)

Example of Human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

反应种属

Human

检测类型

ELISA set

范围

0.15 - 10 ng/mL

检测平台

Reagents

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反应性数据

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产品详情

Human Factor IX Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human Factor IX in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery^® (MSD^®)
Bead-based assays
AlphaLISA^®/AlphaScreen^®
DELFIA^® immunoassays
Simoa^® and Single Molecule Counting (SMC™) immunoassays
Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Factor IX also known as Christmas factor or PTC is an important clotting protein in the coagulation cascade. It is a 415 amino acid protein with a mass of approximately 55 kDa. Factor IX is mainly expressed in the liver and then released into the bloodstream where it plays a significant role in blood clotting processes. The recombinant version of this protein referred to as factor IX recombinant is used therapeutically especially in patients who require coagulation support due to factor deficiencies.

Biological function summary

The function of factor IX involves its role in the amplification phase of the coagulation cascade. It participates as part of the intrinsic tenase complex which forms on negatively charged phospholipids in the presence of calcium ions. This complex significantly enhances the conversion of factor X to its active form factor Xa an important step in the clotting process. The activity of factor IX can be assessed using assays such as the factor IX activity assay and ELISA allowing for precise measurement of its function and quantity.

Pathways

Factor IX is integral within the intrinsic pathway of blood coagulation. It interacts with proteins such as factor VIII and factor X. When activated factor IXa forms a complex with the cofactor factor VIIIa on membrane surfaces intensifying the transformation of factor X to factor Xa which then catalyzes the conversion of prothrombin to thrombin. This series of reactions leads to fibrin formation important for stable clot production. Factor IXa also relates to the pathways influencing cellular signaling and hemostasis.

Factor IX deficiencies cause hemophilia B a genetic disorder that leads to improper blood clotting. This disorder is mainly characterized by spontaneous bleeding or severe bleeding after injury. Patients with hemophilia B usually have mutations in the factor IX gene on the X chromosome affecting the protein's function. Successful treatment often involves replacement therapy using factor IX concentrates or the recombinant form. The interaction with proteases such as factor VIIa highlights its importance in therapeutic strategies for bleeding disorders.

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa (PubMed : 8295821, PubMed : 2592373, PubMed : 20121197, PubMed : 20121198, PubMed : 1730085, PubMed : 19846852, PubMed : 39880037).

See full target information Coagulation factor IX

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人Factor IX (F9)抗体Pair - BSA and Azide free

Human Factor IX (F9) Antibody Pair - BSA and Azide free

Sandwich ELISA - Human Factor IX (F9) Antibody Pair - BSA and Azide free (AB253721)

Sandwich ELISA - Human Factor IX (F9) Antibody Pair - BSA and Azide free (AB253721)