JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB316996

人beta 2 Defensin/BD-2抗体Pair - BSA and Azide free

Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human beta 2 Defensin/BD-2.

查看别名

DEFB102, DEFB2, DEFB4, DEFB4B, DEFB4A, Defensin beta 4A, Beta-defensin 2, Skin-antimicrobial peptide 1, BD-2, hBD-2, SAP1

1 Images
Sandwich ELISA - Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free (AB316996)
  • sELISA

Supplier Data

Sandwich ELISA - Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free (AB316996)

Sandwich ELISA of ab316996 with the capture antibody ab316997 dilution at 2 ug/mL and detector antibody ab316998 dilution at 0.5 ug/mL.

关键信息

反应种属

Human

检测类型

ELISA set

范围

1.56 - 100 pg/mL

检测平台

Reagents

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

style
left-column

规格

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab316996-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human beta 2 Defensin/BD-2 Detector Antibody (unconjugated)", "number":"AB316996-CMP02", "productcode":"AB316998" }, { "size":"1 x 100 µg", "name":"Human beta 2 Defensin/BD-2 Capture Antibody (unconjugated)", "number":"AB316996-CMP01", "productcode":"AB316997" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Beta 2 defensin also known as BD-2 or beta-defensin 2 is a small antimicrobial peptide with a molecular mass of about 4.6 kDa. It plays an important role in the innate immune response by disrupting microbial membranes. BD-2 is expressed in epithelial cells making it important for creating a protective barrier in the skin respiratory tract and urogenital tract. This peptide acts directly against bacteria fungi and some viruses helping to maintain microbial homeostasis in these areas.
Biological function summary

Beta 2 defensin serves as a critical component of host defense mechanisms. It not only shows antimicrobial properties but also influences inflammatory processes. BD-2 interacts with immune cells recruiting and activating them for enhanced defense response. Presently there is no evidence of beta 2 defensin being part of a larger protein complex focusing its function on immediate immune response and cellular signaling in areas it is expressed.

Pathways

Beta 2 defensin engages in critical pathways of immune protection. It is part of the toll-like receptor signaling pathway and engages the NF-kB pathway. These pathways are essential for activating immune responses when pathogens are recognized. Additionally beta 2 defensin interacts with other defensins and related antimicrobial peptides to mount an effective immune response coordinating with various cytokines and chemokines to balance protective actions.

Beta 2 defensin has connections to inflammatory and autoimmune conditions. It has a potential link to psoriasis where its expression levels are increased serving both protective and inflammatory roles. BD-2 is also investigated in cystic fibrosis where its impaired function can contribute to chronic lung infections. In these conditions the altered balance of BD-2 associates it with proteins involved in immune dysregulation contributing to the pathophysiology of these disorders.
style
left-column

产品实验方案

style
left-column

靶点信息

Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria (PubMed : 10837369, PubMed : 9202117). Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM (PubMed : 10837369). Also exhibits antimicrobial activity against the yeast C.albicans (PubMed : 10837369, PubMed : 30050988, PubMed : 9202117). Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death (PubMed : 30050988). Acts as a ligand for C-C chemokine receptor CCR6 (PubMed : 10521347, PubMed : 20068036). Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells, such as immature dendritic cells and memory T cells (PubMed : 10521347, PubMed : 20068036).
See full target information DEFB4A
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB316998

Anti-beta 2 Defensin/BD-2 antibody [EPR26637-70] - BSA and Azide free (Detector)

Recombinant|RabMAb

Sandwich ELISA - Anti-beta 2 Defensin/BD-2 antibody [EPR26637-70] - BSA and Azide free (Detector) (AB316998)

  • 0
  • 0
Proteins & Peptides

AB243124

Recombinant human beta 2 Defensin/BD-2 protein (Active)

Bioactive

SDS-PAGE - Recombinant human beta 2 Defensin/BD-2 protein (Active) (AB243124)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com