Frataxin蛋白Quantity Dipstick Assay试剂盒(ab109881)
概述
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产品名称
Frataxin蛋白Quantity Dipstick Assay试剂盒 -
样品类型
Whole Blood -
检测类型
Sandwich (quantitative) -
种属反应性
与反应: Human -
产品概述
ab109881 is used to rapidly quantify frataxin protein levels from human sample materials. Purification of mitochondria is not necessary for the performance of this assay. Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the mature form of frataxin. One antibody is immobilized on the nitrocellulose membrane of the dipstick in a thin line perpendicular to the length of the dipstick while the other is gold-conjugated which gives a visual signal. When frataxin is present in the sample, a red-colored line appears on the dipstick at the site of the anti-frataxin mAb immobilized on the membrane. The signal intensity is directly related to the level of frataxin in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.
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说明
All components are stable in their provided containers at room temperature out of direct sunlight.
After diluting the 10X Blocking Buffer to 2X, store at 4°C.
For long-term storage, all buffers can be stored at 4°C.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 30 tests Buffer B (10X Blocking solution) 1 x 0.4ml Dipsticks 1 x 30 units Extraction Buffer (ab260490) 1 x 15ml Gold-conjugated antibody (dried in microplate wells) 1 x 30 tests Wash buffer 1 x 2ml -
研究领域
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别名
- FRDA
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数据库链接
- Entrez Gene: 2395 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109881于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent dilution.
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| 说明 |
|---|
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Sandwich ELISA
Use at an assay dependent dilution. |
图片
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Sandwich ELISA - Frataxin Protein Quantity Dipstick Assay Kit (ab109881)An example using ab109881 to quantify frataxin levels from ß -lymphocyte cell culture samples derived from control individuals and Friedreich's Ataxia (FA) patients. Based on the above standard curve values, of protein extract for the controls and FA patient samples. (Note: for a statistical analysis, it is preferred to use two dipsticks for each sample; intra-assay CV's are typically <=10%.) For this analysis, the FA patients had between 550 - 925 GAA repeats on the smaller allele. Using GraphPad software, the signal intensity from the standard curve was interpolated and the relative amount of frataxin in the patients, as compared to the controls, was determined. Based on the above analysis, the patient samples had between 13% and 20% of frataxin levels compared to the control.
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Sandwich ELISA - Frataxin Protein Quantity Dipstick Assay Kit (ab109881)An example using ab109881 to quantify frataxin levels from ß -lymphocyte cell culture samples derived from control individuals and Friedreich's Ataxia (FA) patients. Shown is a 1:2 dilution series using a positive control sample. Approximately 6 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 4 µg of control sample. A one-site hyperbola line was generated for best-fit analysis using GraphPad. -
Sandwich ELISA - Frataxin Protein Quantity Dipstick Assay Kit (ab109881)Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.
数据表及文件
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Datasheet download
文献 (20)
ab109881 被引用在 20 文献中.
- Bouchard C et al. Finding an Appropriate Mouse Model to Study the Impact of a Treatment for Friedreich Ataxia on the Behavioral Phenotype. Genes (Basel) 14:N/A (2023). PubMed: 37628705
- Cherif K et al. Increased Frataxin Expression Induced in Friedreich Ataxia Cells by Platinum TALE-VP64s or Platinum TALE-SunTag. Mol Ther Nucleic Acids 12:19-32 (2018). PubMed: 30195758
- Mikaeili H et al. FAST-1 antisense RNA epigenetically alters FXN expression. Sci Rep 8:17217 (2018). PubMed: 30464193
- Crombie DE et al. Friedreich's ataxia induced pluripotent stem cell-derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency. Aging (Albany NY) 9:1440-1452 (2017). PubMed: 28562313
- Chapdelaine P et al. Development of an AAV9 coding for a 3XFLAG-TALEfrat#8-VP64 able to increase in vivo the human frataxin in YG8R mice. Gene Ther 23:606-14 (2016). Human . PubMed: 27082765
