TripleStain IHC试剂盒: R&R&M on人组织(DAB,AP/Red & Green/HRP) (ab183288)
概述
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产品名称
TripleStain IHC试剂盒: R&R&M on人组织(DAB,AP/Red & Green/HRP) -
检测类型
Polymer Multiplex -
产品概述
Abcam's TripleStain IHC Kit (ab183288) is designed to be used with one mouse and two rabbit primary antibodies, supplied by the user, to detect three distinct antigens on human tissue or cell samples. This kit has been tested on paraffin embedded tissue specimens, however, it can also be used for frozen tissue or cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183288) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit AP Polymer and Rabbit HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and Emerald (green). Abcam's TripleStain IHC Kit (ab183288) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip.
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经测试应用
适用于: IHC-Fr, IHC-Pmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 6 ml 18 ml 60 ml Antibody Blocker (40x) 1 x 30ml 1 x 50ml 1 x 100ml Blocker A 1 x 6ml 1 x 18ml 1 x 60ml Blocker B 1 x 6ml 1 x 18ml 1 x 60ml DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml DAB Substrate 1 x 12ml 1 x 36ml 1 x 120ml Emerald Chromogen 1 x 6ml 1 x 18ml 1 x 60ml Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Non-aqueous Mounting Medium 1 x 6ml 1 x 18ml 0 x 0ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 1 x 24ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate 1 x 15ml 1 x 36ml 1 x 120ml Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Rabbit HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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