TripleStain IHC试剂盒: M&R&Rt on rodent组织(DAB,HRP/Green & AP/Red) (ab183297)
概述
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产品名称
TripleStain IHC试剂盒: M&R&Rt on rodent组织(DAB,HRP/Green & AP/Red) -
检测类型
Polymer Multiplex -
产品概述
The TripleStain IHC kit (ab183297) is designed to be used with user supplied mouse, rat and rabbit primary antibodies to detect three distinct antigens on mouse tissue and cell samples or human tissue. This kit has been tested on paraffin embedded tissue, however it can also be used on frozen tissue or cell smears.
Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens and their co-expression in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-rat IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. This kit has been optimized to have no cross detection when detecting two primary antibodies from the mouse and rat host species using a unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6-7 hours (with antigen retrieval). This protocol also includes a method to dehydrate, clear and permanently mount slides with coverslip.
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经测试应用
适用于: IHC-Fr, IHC-Pmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 60 ml 6 ml 18 ml Antibody Blocker (40x) 1 x 100ml 1 x 30ml 1 x 50ml DAB Chromogen (20x) 1 x 6ml 1 x 1.5ml 1 x 2ml DAB Substrate 1 x 120ml 1 x 12ml 1 x 36ml Emerald Chromogen 1 x 60ml 1 x 6ml 1 x 18ml Mouse HRP Polymer 1 x 60ml 1 x 6ml 1 x 18ml Non-aqueous Mounting Medium 0 x 0ml 1 x 6ml 1 x 18ml Permanent Red Activator (5x) 1 x 24ml 1 x 3ml 1 x 7.2ml Permanent Red Chromogen (100x) 1 x 1.2ml 1 x 150µl 1 x 360µl Permanent Red Substrate 1 x 120ml 1 x 15ml 1 x 36ml Rabbit HRP Polymer 1 x 60ml 1 x 6ml 1 x 18ml Rat AP Polymer 1 x 60ml 1 x 6ml 1 x 18ml Rat Primer 1 x 60ml 1 x 6ml 1 x 18ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183297于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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