TripleStain IHC试剂盒: M&R&G on人组织(DAB,AP/Red & HRP/Green) (ab183290)
概述
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产品名称
TripleStain IHC试剂盒: M&R&G on人组织(DAB,AP/Red & HRP/Green) -
检测类型
Polymer Multiplex -
产品概述
Abcam's TripleStain IHC Kit (ab183290) is designed to be used with user-supplied one goat, one mouse and one rabbit primary antibodies to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissue; however it may also be used on frozen or freshly prepared monolayer cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183290) supplies polymer enzyme conjugates: Goat HRP Polymer, Rabbit AP Polymer and Mouse HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and Emerald (green). Abcam's TripleStain IHC Kit (ab183290) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross reaction when detecting three different primary antibodies using our unique blocking system. Simplified steps allow the user to complete triple staining within 3 hours (without antigen retrieval) or 4 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with a coverslip.
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经测试应用
适用于: IHC-P, IHC-Frmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 60 ml 18 ml 6 ml 240 ml DAB Chromogen (20x) 1 x 6ml 1 x 2ml 1 x 1.5ml 1 x 6ml DAB Substrate 1 x 120ml 1 x 36ml 1 x 12ml 1 x 120ml Emerald Chromogen 1 x 60ml 1 x 18ml 1 x 6ml 1 x 120ml Goat HRP Polymer 1 x 60ml 1 x 18ml 1 x 6ml 1 x 60ml Mouse HRP Polymer 1 x 60ml 1 x 18ml 1 x 6ml 1 x 120ml Non-aqueous Mounting Medium 0 x 0ml 1 x 18ml 1 x 6ml 2 x 30ml Permanent Red Activator (5x) 1 x 24ml 1 x 7.2ml 1 x 3ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 1.2ml 1 x 360µl 1 x 150µl 1 x 1.2ml Permanent Red Substrate 1 x 120ml 1 x 36ml 1 x 15ml 1 x 120ml Rabbit AP Polymer 1 x 60ml 1 x 18ml 1 x 6ml 1 x 60ml Triple Stain Blocker 1 x 60ml 1 x 18ml 1 x 6ml 1 x 120ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab183290 被引用在 1 文献中.
- D S P et al. Fused toes homolog, a potential molecular regulator of human papillomavirus type 16 E6 and E7 oncoproteins in cervical cancer. PLoS One 17:e0266532 (2022). PubMed: 35421154