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StayRed/AP 30ml (Alcohol & Xylene Compatible) (ab103741)

Submit a review Q&A (4)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - StayRed/AP 30ml (Alcohol & Xylene Compatible) (ab103741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - StayRed/AP 30ml (Alcohol & Xylene Compatible) (ab103741)

概述

  • 产品名称

    StayRed/AP 30ml (Alcohol & Xylene Compatible)

  • 检测类型

    Direct

  • 产品概述

    StayRed/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayRed/AP produces a brilliant dark red color. It is also insoluble in organic solvents so sections can be dehydrated in alcohol, cleared in xylene (or a xylene substitute) and permenently mounted.

  • 说明

    Intended Use:

    Substrate/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.

     

    Working Solution:

    Aliquot 3mL of StayRed/AP Substrate Buffer in a mixing bottle. Add one drop (~20μL) of StayRed/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.

    Note: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.

     

    Procedure:

    • After SA-alkaline phosphatase incubation, wash tissue sections with wash buffer.
    • Wipe slides, removing excess buffer. Add enough drops of StayRed/AP working solution to cover tissue sections.
    • Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under microscope for signal development. Once desired signal to noise ratio is achieved, stop reaction by rinsing slides in wash buffer.
    • Counterstain sections in hematoxylin.
    • Dehydrate sections in alcohol, clear in xylene (or xylene-substitute), and mount in permanent mounting medium.

性能

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - StayRed/AP 30ml (Alcohol & Xylene Compatible) (ab103741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - StayRed/AP 30ml (Alcohol & Xylene Compatible) (ab103741)

    Formalin fixed paraffin embedded Human tonsil stained with HMW Cytokeratin antibody (ab850) labeled with PermaRed/AP Chromogen (ab103741) produces a brilliant red color.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - StayRed/AP 30ml (Alcohol & Xylene Compatible) (ab103741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - StayRed/AP 30ml (Alcohol & Xylene Compatible) (ab103741)
    StayRed staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab103741？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab103741 被引用在 2 文献中.

  • Aznar MA  et al. Immunotherapeutic effects of intratumoral nanoplexed poly I:C. J Immunother Cancer 7:116 (2019). PubMed: 31046839
  • Shami A  et al. Fibromodulin deficiency reduces low-density lipoprotein accumulation in atherosclerotic plaques in apolipoprotein E-null mice. Arterioscler Thromb Vasc Biol 33:354-61 (2013). PubMed: 23202368

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Question

Inquiry: Hi there, I was wondering whether you can help. I have to stain mouse tissue using an unconjugated primary antibody (Anti-Mouse/Rat MULT1, Host - Armenian Hamster IgG) and a biotinylated secondary one (Anti-Armenian Hamster IgG Biotin, Host - Goat IgG). I have to perform IHC staining. Could you please advise me which of your detection kits I could use for this purpose? Thank you very much for your help. Kind regards,

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Answer

Thank you for your inquiry.

I can suggest to use a strepavidin that is coupled to an enzyme, for example: ab128985 (Streptavidin Peroxidase (Ready-To-Use)) and combine this with a suitable chromogen, for example: ab94665 (DAB substrate kit)

https://www.abcam.com/Streptavidin-Peroxidase-Ready-To-Use-ab128985.html (or use the following: https://www.abcam.com/Streptavidin-Peroxidase-Ready-To-Use-ab128985.html).

https://www.abcam.com/DAB-substrate-kit-ab94665.html (or use the following: https://www.abcam.com/DAB-substrate-kit-ab94665.html).

Another possible combination would be a strepavidin-AP ab128984 (Streptavidin Alkaline Phosphatase (Ready-To-Use)) with a more colourful chromogen like ab103743 (StayBlue/AP 30ml), ab156428 (StayGreen/AP) or ab103741 (StayRed/AP 30ml).

https://www.abcam.com/Streptavidin-Alkaline-Phosphatase-Ready-To-Use-ab128984.html (or use the following: https://www.abcam.com/Streptavidin-Alkaline-Phosphatase-Ready-To-Use-ab128984.html).

https://www.abcam.com/StayBlue-AP-30ml-Alcohol-and-Xylene-Substitute-Compatible-ab103743.html (or use the following: https://www.abcam.com/StayBlue-AP-30ml-Alcohol-and-Xylene-Substitute-Compatible-ab103743.html).

https://www.abcam.com/StayGreenAP-Plus-Alcohol-and-Xylene-Substitute-Compatible-ab156428.html (or use the following: https://www.abcam.com/StayGreenAP-Plus-Alcohol-and-Xylene-Substitute-Compatible-ab156428.html).

https://www.abcam.com/StayRedAP-30ml-Alcohol--Xylene-Compatible-ab103741.html (or use the following: https://www.abcam.com/StayRedAP-30ml-Alcohol--Xylene-Compatible-ab103741.html).

I hope this information is helpful and wish you good luck with your research.

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Question

Hello,
I am interested in your product StayGreen and StayRed substrates for immunohistochemistry. I am currently planning to do double immunohistochemistry and are looking into what colors that would be good together. To combine with a DAB staining would you recommend StayGreen or StayRed? Do you have any experience of staining for two surface markers and what substrates that would be good to use in such assay? I want to be able to use hematoxylin as a counterstaining.
Best regards

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Answer


The source of StayRed and StayBlue recommend to do sequential staining. Their recommendations on chromogens for double staining are DAB and StayRed (https://www.abcam.com/ab103741). In order to use these chromogens first use DAB, then RED, followed by counter stain ex: Hematoxylin.

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Question

To abcam, We purchased the stayred/AP ab103741, and have made some stainings.  We wanted to use this product as it it is compatible with alcohol and xylene.  But I made double stainings on Friday, vwf with DAB and alpha smooth muscle with stayred. The DAB staining still looks perfect, but all the red colour is gone over the week end, as if it has dissolved, and the staining looked just perfect on Friday. I have after the staining, counterstained with heamatoxylene, rinsed in water, and dehydrated in increasing percentage of alcohol till 99,9 % alcohol and  mounted with glass slides using Eukitt mounting media. Could you tell me,  what has happened,  I have used the stayred for other stainings earlier, and it looks as if the red colour is starting to dissolve. Best regards        

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Answer

Thank you for your enquiry and your interest in our products. I can confirm that this product (ab103741) works as double stain with HRP/DAB and it is suitable with Alkaline phosphatase detection. It may be worth first developing DAB and then Red since StayRed/AP can go through Xylene and alcohol without having any issues. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question

Could the StayRed and StayGreen chromegen's be used on the same tissue as a sort on non-fluorescent double-labelling? Thanks! Amanda

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Answer

Thank you for your enquiry. I can confirm that these products can be used together for double staining. However, I can recommend to consider the following: 1. We would suggest to ensure the primary antibodies used in the stains undergo the same conditions for antigen/epitope retrieval. 2. Since the StayRed is a permanent Chromogen (i.e. both alcohol and xylene insoluble) it must be used as the first Chromogen in the double staining protocol. 3. The StayGreen is also alcohol insoluble, however since it is soluble in xylene it must be used with a xylene substitute. The tissue can then be mounted permanently. I was hoping to obtain any images of double staining for you, but regrettably our source does not have these available. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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