DoubleStain IHC试剂盒: G&M on人组织(BCIP & AEC) (ab183271)
概述
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产品名称
DoubleStain IHC试剂盒: G&M on人组织(BCIP & AEC) -
检测类型
Polymer Multiplex -
产品概述
The DoubleStain IHC kit (ab183271) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples. This kit has been tested in paraffin tissues, however it can also be used on frozen specimens and freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry for the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit supplies two polymer enzyme conjugates: AP polymer anti-Goat IgG and HRP(AEC) polymer anti-Mouse IgG with two distinct substrates/chromogens, BCIP/NBT (Purple) and AEC (Red). Users will apply the two enzyme conjugates onto the specimen sequentially. If only the anti-goat antigen is present only the BCIP/NBT(Purple) will be present and if the mouse antigen is present only the AEC(Red) will be present. Abcam's DoubleStain IHC Kit is a non-biotin system avoiding endogenous biotin non-specific binding.
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经测试应用
适用于: IHC-Fr, IHC-Pmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 18 ml 6 ml 60 ml AEC Chromogen (20x) 1 x 4ml 1 x 2ml 1 x 8ml AEC Substrate (20x) 1 x 2ml 1 x 1ml 1 x 4ml Aqueous Mounting Medium 1 x 18ml 1 x 6ml 1 x 60ml DoubleStain Blocker 1 x 18ml 1 x 6ml 1 x 60ml Goat AP Polymer 1 x 18ml 1 x 6ml 1 x 60ml Hydrogen Peroxide (20x) 1 x 2ml 1 x 1ml 1 x 4ml Mouse HRP (AEC) Polymer 1 x 18ml 1 x 6ml 1 x 60ml Ready to Use BCIP/NBT 1 x 18ml 1 x 6ml 1 x 60ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183271于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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