DoubleStain CL IHC试剂盒: G&Rt on人/小鼠组织(HRP/Green & AP/Red) (ab183282)
概述
-
产品名称
DoubleStain CL IHC试剂盒: G&Rt on人/小鼠组织(HRP/Green & AP/Red) -
检测类型
Polymer Multiplex -
产品概述
Abcam's Doublestain IHC Kit (ab183282) is designed to be used with user supplied goat and rat primary antibodies to detect two distinct antigens on human or mouse tissue or cell samples. The kit has been tested on paraffin–embedded human and mouse tissues.
Double staining is one of most common methods used in immunohistostaining allowing for the detection of two distinct antigens in a single tissue. Abcam's Doublestain IHC Kit (ab183282) supplies the user with two polymer enzyme conjugates: Goat AP Polymer and Rat HRP Polymer with two distinct substrates/chromogens, Emerald and Permanent Red. With this protocol the goat and rat primary antibodies will be applied to the tissue at the same time but the user will sequentially apply the polymer enzyme conjugate to the specimen. When two proteins are present in the same location a blue or purple color will develop depending on the quantity of each antigen screened. If only the anti-goat antigen is present only the Permanent Red chromogen will be visible and if only the Rat antigen is present then only the Emerald chromogen will be seen. Abcam's Doublestain IHC Kit (ab183282) is a non-biotin system avoiding endogenous biotin non-specific binding.
-
经测试应用
适用于: IHC-P, IHC-Frmore details
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 18 ml 6 ml 60 ml DoubleStain Blocker 1 x 18ml 1 x 6ml 1 x 60ml Emerald Chromogen 1 x 18ml 1 x 6ml 1 x 60ml Goat AP Polymer 1 x 18ml 1 x 6ml 1 x 60ml Non-aqueous Mounting Medium 1 x 18ml 1 x 6ml 0 x 0ml Permanent Red Activator (5x) 1 x 7.2ml 1 x 3ml 1 x 14ml Permanent Red Chromogen (100x) 1 x 360µl 1 x 150µl 1 x 700µl Permanent Red Substrate 1 x 36ml 1 x 15ml 1 x 120ml Rat HRP Polymer 1 x 18ml 1 x 6ml 1 x 60ml Rat Primer 1 x 18ml 1 x 6ml 1 x 60ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183282于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
说明 |
---|
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab183282 尚未被引用在任何文献中。