AB64034

大鼠TNF alpha ELISPOT试剂盒

Name: 大鼠TNF alpha ELISPOT Kit (ab64034)| Abcam中文官网
Brand: Abcam Limited
SKU: AB64034
Price: 4793 CNY
Availability: InStock

Rat TNF alpha ELISPOT Kit

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Rat TNF alpha ELISPOT Kit is a ELISPOT kit for the sensitive detection of Rat TNF alpha production in a single cell suspension.

查看别名

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

关键信息

检测方法

Colorimetric

样品类型

Suspension cells

反应种属

Rat

检测类型

Sandwich (qualitative)

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISPOT": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Principal

After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

Recognizes natural rat TNF alpha

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规格

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab64034-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"TNFα Biotinylated detection antibody", "number":"AB64034-CMP02" }, { "size":"1 x 50 mL", "name":"Ready-to-use BCIP/NBT substrate buffer", "number":"AB64034-CMP05" }, { "size":"1 x 1 g", "name":"Bovine Serum Albumin", "number":"AB64034-CMP01" }, { "size":"5 x 1 Unit", "name":"Rat TNFα Pre-coated 96-well PDVF-bottomed plates", "number":"AB64034-CMP04" }, { "size":"1 x 50 µL", "name":"Streptavidin - Alkaline Phosphatase conjugated", "number":"AB64034-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab64034-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"TNFα Biotinylated detection antibody", "number":"AB64034-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"Streptavidin - Alkaline Phosphatase conjugated", "number":"AB64034-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Rat TNFα Pre-coated 96-well PDVF-bottomed plates", "number":"AB64034-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Ready-to-use BCIP/NBT substrate buffer", "number":"AB64034-CMP05", "productcode":"" }, { "size":"1 x 200 mg", "name":"Bovine Serum Albumin", "number":"AB64034-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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产品实验方案

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靶点信息

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

See full target information Tnf

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Abcam Product Promise

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大鼠TNF alpha ELISPOT试剂盒

Rat TNF alpha ELISPOT Kit