小鼠Interferon gamma ELISPOT试剂盒(ab64029)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Colorimetric
- Sample type: Suspension cells
- Reacts with: Mouse
概述
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产品名称
小鼠Interferon gamma ELISPOT试剂盒
参阅全部 Interferon gamma 试剂盒 -
检测方法
Colorimetric -
样品类型
Suspension cells -
检测类型
Sandwich (qualitative) -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principle
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural murine Interferon gamma.
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经测试应用
适用于: ELISpotmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 5 x 96 tests Bovine Serum Albumin 1 x 2g 1 x 2g IFNγ Biotinylated detection antibody 1 x 100µl 1 vial Mouse IFNγ Pre-coated 96-well PVDF-bottomed plates 1 unit 5 units Ready-to-use BCIP/NBT substrate buffer 1 x 11ml 2 x 25ml Streptavidin - Alkaline Phosphatase conjugated 1 x 10µl 1 x 50µl -
研究领域
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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别名
- IF 1
- IFG
- IFI
see all -
数据库链接
- Entrez Gene: 15978 Mouse
- SwissProt: P01580 Mouse
- Unigene: 240327 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64029于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
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| 说明 |
|---|
|
ELISpot
Use at an assay dependent dilution. |
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab64029 被引用在 8 文献中.
- Tregoning JS et al. Formulation, inflammation, and RNA sensing impact the immunogenicity of self-amplifying RNA vaccines. Mol Ther Nucleic Acids 31:29-42 (2023). PubMed: 36589712
- Fu Y & Xiong S Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism. PLoS Pathog 19:e1011090 (2023). PubMed: 36634130
- Hoang HD et al. Adaptation of transgene mRNA translation boosts the anticancer efficacy of oncolytic HSV1. J Immunother Cancer 11:N/A (2023). PubMed: 36958764
- Tai W et al. An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variants. Nat Commun 14:2962 (2023). PubMed: 37221158
- Wang Z et al. Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice. Mol Ther Nucleic Acids 34:102045 (2023). PubMed: 37876532
- Chen J et al. Lipid nanoparticle-mediated lymph node-targeting delivery of mRNA cancer vaccine elicits robust CD8+ T cell response. Proc Natl Acad Sci U S A 119:e2207841119 (2022). PubMed: 35969778
- Zhou S et al. FAP-Targeted Photodynamic Therapy Mediated by Ferritin Nanoparticles Elicits an Immune Response against Cancer Cells and Cancer Associated Fibroblasts. Adv Funct Mater 31:N/A (2021). PubMed: 35822179
- Liu Z et al. Detecting Tumor Antigen-Specific T Cells via Interaction-Dependent Fucosyl-Biotinylation. Cell 183:1117-1133.e19 (2020). PubMed: 33096019