人IL-17酶联免疫斑点法（ELISPOT）Set (ab83712)
Key features and details
- Assay type: Sandwich (qualitative)
- Sample type: Suspension cells
- Reacts with: Human
概述
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产品名称
人IL-17酶联免疫斑点法（ELISPOT）Set
参阅全部 IL-17 试剂盒 -
样品类型
Suspension cells -
检测类型
Sandwich (qualitative) -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Intended Use
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation. The ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principle of Method
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural human IL-17A.
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经测试应用
适用于: ELISpotmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 5 x 96 tests 10 x 96 tests Bovine Serum Albumin 1 x 1g 2 x 1g Human IL-17 Biotinylated detection antibody 1 vial 1 vial Human IL-17 Capture antibody 1 x 500µl 2 x 500µl Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl -
研究领域
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相关性
IL-17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8 and GCSF production in fibroblasts; the enhancement of surface expression of ICAM 1 in fibroblasts; activation of NF kappaB and costimulation of T cell proliferation. IL-17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL-17 consists of 155 amino acids. To generate the mature IL-17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL-17 shows approximately 62.5% amino acid homology to mouse IL-17 and 58% amino acid homology to rat IL-17. -
细胞定位
Secreted -
别名
- CTLA 8
- CTLA8
- Cytotoxic T lymphocyte associated antigen 8
see all -
数据库链接
- Entrez Gene: 3605 Human
- Omim: 603149 Human
- SwissProt: Q16552 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab83712于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
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| 说明 |
|---|
|
ELISpot
Use at an assay dependent dilution. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab83712 被引用在 1 文献中.
- Dissanayake K et al. Potential applicability of cytokines as biomarkers of disease activity in rheumatoid arthritis: Enzyme-linked immunosorbent spot assay-based evaluation of TNF-a, IL-1ß, IL-10 and IL-17A. PLoS One 16:e0246111 (2021). PubMed: 33497394