人Fas Ligand ELISPOT试剂盒(ab62920)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Colorimetric
- Sample type: Suspension cells
- Reacts with: Human
概述
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产品名称
人Fas Ligand ELISPOT试剂盒
参阅全部 Fas Ligand 试剂盒 -
检测方法
Colorimetric -
样品类型
Suspension cells -
检测类型
Sandwich (qualitative) -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principal:
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural human Fas Ligand.
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经测试应用
适用于: ELISpotmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 5 x 96 tests Bovine Serum Albumin 1 x 200mg 1 x 1g Fas Ligand Biotinylated detection antibody 1 vial 1 vial Fas Ligand Pre-coated 96-well plate 1 unit 5 units Ready-to-use BCIP/NBT substrate buffer 1 x 10ml 1 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 10µl 1 x 50µl -
研究领域
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相关性
The protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with Fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus. -
细胞定位
Cell membrane; single-pass type II membrane protein. Secreted protein. May be released into the extracellular fluid, probably by cleavage form the cell surface. -
别名
- Apoptosis antigen ligand
- APT1LG1
- APTL
see all -
数据库链接
- Entrez Gene: 356 Human
- SwissProt: P48023 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62920于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
|
| 说明 |
|---|
|
ELISpot
Use at an assay dependent dilution. |
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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