大鼠Leptin ELISA试剂盒(ab100773)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 10.97 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠Leptin ELISA试剂盒
参阅全部 Leptin 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 30 pg/ml -
范围
10.97 pg/ml - 8000 pg/ml -
回收率
84 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 75.43 68% - 89% Serum 92.32 83% - 102% Plasma 84.25 75% - 94% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s Leptin Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Leptin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rat Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Rat Leptin 2 vials Leptin Microplate (12 x 8 wells) 1 unit Recombinant rat Leptin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
疾病相关
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
序列相似性
Belongs to the leptin family. -
细胞定位
Secreted. - Information by UniProt
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别名
- FLJ94114
- LEP
- LEP_HUMAN
see all -
数据库链接
- Entrez Gene: 25608 Rat
- SwissProt: P50596 Rat
- Unigene: 44444 Rat
图片
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Sandwich ELISA - ab100773 Leptin Rat ELISA Kit
Rat Leptin measured in supernatanses showing quantity (ng) per mL of tested sample
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Sandwich ELISA - ab100773 Leptin Rat ELISA KitRat Leptin measured in biofluids showing quantity (ng) per mL of tested sample
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Sandwich ELISA - ab100773 Leptin Rat ELISA KitStandard curve: mean of duplicates (+/-SD) with background readings subtracted
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ELISA - Leptin Rat ELISA Kit (ab100773)
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab100773 被引用在 22 文献中.
- Vega-Torres JD et al. Short-term exposure to an obesogenic diet during adolescence elicits anxiety-related behavior and neuroinflammation: modulatory effects of exogenous neuregulin-1. Transl Psychiatry 12:83 (2022). PubMed: 35220393
- Liu X et al. Decrease in leptin mediates rat bone metabolism impairments during high-fat diet-induced catch-up growth by modulating the OPG/RANKL balance. 3 Biotech 11:103 (2021). PubMed: 33552831
- Chen J et al. Roux-en-Y gastric bypass contributes to weight loss-independent improvement in hypothalamic inflammation and leptin sensitivity through gut-microglia-neuron-crosstalk. Mol Metab 48:101214 (2021). PubMed: 33741533
- Hankir MK et al. Leptin Receptors Are Not Required for Roux-en-Y Gastric Bypass Surgery to Normalize Energy and Glucose Homeostasis in Rats. Nutrients 13:N/A (2021). PubMed: 34064308
- Strzelewicz AR et al. Interactive effects of compounding multidimensional stressors on maternal and male and female rat offspring outcomes. Horm Behav 134:105013 (2021). PubMed: 34171577