大鼠IFN gamma ELISA试剂盒(ab46107)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠IFN gamma ELISA试剂盒
参阅全部 Interferon gamma 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 19 365.1pg/ml 12.7 2.9% B 20 554.6pg/ml 16.4 3.4% 批次间 样品 n Mean SD CV% A 6 148.2pg/ml 6.6 4.5% B 6 560.7pg/ml 29.5 5.2% -
样品类型
Cell culture supernatant, Serum -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s IFN gamma Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in cell culture supernatants, buffered solutions and plasma.
A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.
This kit will recognize both endogenous and recombinant rat IFN gamma
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-IFN gamma Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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别名
- IF 1
- IFG
- IFI
see all -
数据库链接
- Entrez Gene: 25712 Rat
- SwissProt: P01581 Rat
- Unigene: 10795 Rat
图片
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Typical Standard Curve
Representative Standard Curve using ab46107
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab46107 被引用在 13 文献中.
- Mohamed ME et al. The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NF?B Pathway. Antioxidants (Basel) 11:N/A (2022). PubMed: 35326185
- Yang W et al. Therapeutic efficacy of chitosan nanoparticles loaded with BCG-polysaccharide nucleic acid and ovalbumin on airway inflammation in asthmatic mice. Eur J Clin Microbiol Infect Dis 40:1623-1631 (2021). PubMed: 33666790
- Wang J et al. HDAC inhibitor sodium butyrate prevents allergic rhinitis and alters lncRNA and mRNA expression profiles in the nasal mucosa of mice. Int J Mol Med 45:1150-1162 (2020). PubMed: 32124940
- Li Y et al. Long noncoding RNA colorectal neoplasia differentially expressed alleviates sepsis-induced liver injury via regulating miR-126-5p. IUBMB Life 72:440-451 (2020). PubMed: 32031750
- Yan M et al. The Mechanism of Electroacupuncture at Zusanli Promotes Macrophage Polarization during the Fibrotic Process in Contused Skeletal Muscle. Eur Surg Res 60:196-207 (2019). PubMed: 31694021