大鼠GM-CSF ELISA试剂盒(ab100762)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 12.35 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠GM-CSF ELISA试剂盒
参阅全部 GM-CSF 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
12.35 pg/ml - 3000 pg/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 90.53 81% - 101% Serum 136.4 128% - 143% Plasma 118.8 88% - 136% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s GM-CSF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat GM-CSF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rat GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti- rat GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Rat GM-CSF 2 vials GM-CSF Microplate (12 x 8 wells) 1 unit Recombinant Rat GM-CSF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. -
序列相似性
Belongs to the GM-CSF family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
see all -
数据库链接
- Entrez Gene: 116630 Rat
- SwissProt: P48750 Rat
- Unigene: 44285 Rat
图片
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Typical Standard Curve
Representative standard curve using ab100762
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Typical Standard CurveRepresentative standard curve using ab100762
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab100762 尚未被引用在任何文献中。