Key features and details
- Sensitivity: 5 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
参阅全部 CX3CL1 试剂盒
样品类型Cell culture extracts, Tissue Extracts
灵敏度< 5 pg/ml
范围2.74 pg/ml - 2000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture extracts 93.54 84% - 104% Tissue Extracts 92.87 83% - 103%
实验步骤Multiple steps standard assay
Abcam’s Fractalkine Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fractalkine in cell lysates and tissue lysates.
This assay employs an antibody specific for Rat Fractalkine coated on a 96- well plate. Standards and samples are pipetted into the wells and Fractalkine present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fractalkine antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fractalkine bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The standard is affinity purified.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 240X HRP-Streptavidin Concentrate 1 x 200µl 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-Rat Fractalkine 2 vials Fractalkine Microplate (12 x 8 wells) 1 unit Recombinant Rat Fractalkine Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
相关性CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.
- A 152E5.2
- ABCD 3
ab100761 被引用在 1 文献中.
- Huang X et al. Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-?B. Mediators Inflamm 2017:9734837 (2017). ELISA ; Rat . PubMed: 28634422