Key features and details
- Sensitivity: 20 pg/ml
- Range: 24.69 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
参阅全部 CXCL5 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 20 pg/ml
范围24.69 pg/ml - 6000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 93.53 85% - 103% Serum 88.63 83% - 102% Plasma 89.45 84% - 103%
实验步骤Multiple steps standard assay
Abcam’s CXCL5 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat CXCL5 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rat CXCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Rat CXCL5 2 vials CXCL5 Microplate (12 x 8 wells) 1 unit Recombinant Rat CXCL5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
相关性CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
- AMCF II
- C X C motif chemokine 5