为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Developmental proteins ELISA kits

大鼠CNTF ELISA试剂盒(ab100758)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (4)References (1)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 10 pg/ml
  • Range: 8.23 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

你可能会感兴趣

一抗
Product image
Anti-Myelin Basic Protein antibody [EPR21188] (ab218011)
ELISA
Product image
Rat MIP2 ELISA Kit (CXCL2) (ab282308)

查看更多相关产品

概述

  • 产品名称

    大鼠CNTF ELISA试剂盒
    参阅全部 CNTF 试剂盒
  • 检测方法

    Colorimetric
  • 样品类型

    Cell culture supernatant, Serum, Plasma
  • 检测类型

    Sandwich (quantitative)
  • 灵敏度

    < 10 pg/ml
  • 范围

    8.23 pg/ml - 2000 pg/ml
  • 回收率

    100 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 97.3 89% - 108%
    Serum 105.6 93% - 112%
    Plasma 104.2 92% - 110%
  • 实验步骤

    Multiple steps standard assay
  • 种属反应性

    与反应: Rat
  • 产品概述

    Abcam’s CNTF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat CNTF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 平台

    Microplate

性能

  • 存放说明

    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-rat CNTF 2 vials
    CNTF Microplate (12 strips x 8 wells) 1 unit
    Recombinant rat CNTF Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究领域

    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Developmental proteins ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
    • Neuroscience
    • Development
  • 相关性

    Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.
  • 细胞定位

    Cytoplasmic
  • 别名

    • AI429687
    • Ciliary Neurotrophic Factor
    • HCNTF
    • MGC41235
    • OTTHUMP00000174731
    • OTTMUSP00000029848
    see all
  • 数据库链接

    • Entrez Gene: 25707 Rat
    • SwissProt: P20294 Rat

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab100758

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab100758

    实验方案

    • Protocol Booklet

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab100758?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab100758 被引用在 1 文献中.

    • Du Z  et al. Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression. J Cell Physiol 231:1301-12 (2016). PubMed: 26516696

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    Abreview for CNTF Rat ELISA Kit

    Average Average 3/5 (Ease of Use)
    Abreviews
    Abreviews

    Sample: Mouse Serum (blood)
    Specification: blood
    Amount of Sample: 100 µg
    Type of Kit Used: ELISA
    Positive control: rat serum
    purified CNTF
    Did you use the Abcam Kit protocol?: Yes

    Additional data
    Additional Notes: Wanted to use the kit to measure CNTF in mouse serum, but it did not work for the mouse samples. Mouse serum was used in multiple amounts, but the plate wells containing the mouse samples were no different than background. The kit worked well with serial dilutions of my positive control (rat serum), though. The kit is otherwise easy to use.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 21 2011

    Question

    I have used the CNTF Rat ELISA Kit (ab100758). Can you tell me which part of the CNTF protein the CNTF Ab recognize?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 28 2013

    Answer

    Epitope mapping has not been performed so the exact binding sequence that the antibody pair recognizes is not known. All that is known is that the antibodies are binding somewhere on the full length protein (http://www.uniprot.org/uniprot/P20294).

    Read More

    Elisa Thomas

    Abcam Scientific Support

    回复于 Aug 28 2013

    Question

    Will this ELISA kit detect the mouse protein?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2011

    Answer

    Thank you for your inquiry.  Unfortunately, we have not tested mouse samples with the ab99994 kit or ab100758 kit. The lab didn't think that the human kit ab99994 would detect mouse samples since it didn't work in rat (the mouse and rat proteins are 95% homologous). Considering all this, there is a chance that the rat kit ab100758 may recognize the mouse protein, since the proteins are so similar.  If you would like to test ab100758 in mouse, let me know and we can set you up with a testing discount.  If you purchase the kit, test it in mouse, and it does or does not work, we would reward you with a free primary antibody or that price off another ELISA kit after you submit an Abreview to us with your data.  Please let me know if you would like to test ab100758 in mouse and I will set you up in our system before you purchase it.  I look forward to your reply.    

    Read More

    Abcam Scientific Support

    回复于 Dec 20 2011

    Question

    Will either of these CNTF ELISA kits possibly cross react with mouse CNTF?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2011

    Answer

    Thank you for your email.  I have addressed your question below. ab100758 : No cross reactivity data is available testing mouse samples with this rat kit therefore the only way to know would be to test and see.  However, both the capture and detection antibody are polyclonal, and since rat and mouse CNTF are 95% identical at the amino acid level, it is quite likely this kit will work with mouse samples. If you are interested in testing this kit in mouse samples, please see below on how to get a free antibody or free kit for testing this kit with your mouse samples: =================== Thank you very much for your interest in ab100758: To our knowledge, ab100758 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab100758 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free kit OR 1 free antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab100758 in mouse. I will then send a discount code. This code must be issued before purchasing ab(XXX) so please wait for my reply before ordering. 2. Purchase ab100758 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for 1 free kit OR 1 free antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab100758 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.      

    Read More

    Abcam Scientific Support

    回复于 Nov 21 2011

    Question

            OK...I would be willing to try the kit and submit a review for the discount later on...please send the code so that I can order. 

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2011

    Answer

    I am very pleased to hear you would like to accept our offer and test ab100758 in mouse. This code will give you 1 free kit OR 1 free antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

    Read More

    Abcam Scientific Support

    回复于 Nov 21 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有