大鼠CD62L ELISA试剂盒(Selectin L) (ab100776)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠CD62L ELISA试剂盒(Selectin L)
参阅全部 CD62L 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 6 pg/ml -
范围
8.23 pg/ml - 6000 pg/ml -
回收率
96 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 98.42 89% - 107% Serum 97.06 86% - 107% Plasma 94.33 85% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s CD62L Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CD62L in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for CD62L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CD62L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti Rat CD62L 2 vials CD62L Microplate (12 x 8 wells) 1 unit Recombinant rat CD62L Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. -
细胞定位
Cell Membrane; single-pass type I membrane protein. -
别名
- A.11
- AI528707
- CD62 antigen ligand
see all -
数据库链接
- Entrez Gene: 29259 Rat
- SwissProt: Q63762 Rat
图片
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Typical standard curve
Representative standard curve using ab100776
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Typical standard curveRepresentative standard curve using ab100776
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab100776 尚未被引用在任何文献中。