大鼠C Reactive蛋白ELISA试剂盒(PTX1) (ab108827)
Key features and details
- Sensitivity: 0.25 ng/ml
- Range: 0.391 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠C Reactive蛋白ELISA试剂盒(PTX1)
参阅全部 C Reactive Protein 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3.4% 批次间 样品 n Mean SD CV% Overall 8.5% -
样品类型
Cell culture supernatant, Urine, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.25 ng/ml -
范围
0.391 ng/ml - 25 ng/ml -
回收率
95 %
-
检测时间
3h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s C Reactive Protein (PTX1) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein in urine, plasma, serum, and cell culture supernatants.
A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Rat C Reactive Protein Antibody 1 x 120µl C Reactive Protein Microplate (12 x 8 well strips) 1 unit C Reactive Protein Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
组织特异性
Found in plasma. -
序列相似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
细胞定位
Secreted. - Information by UniProt
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别名
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
数据库链接
- Entrez Gene: 25419 Rat
- SwissProt: P48199 Rat
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab108827 被引用在 18 文献中.
- Kalinich JF et al. Serum Indicators of Oxidative Damage from Embedded Metal Fragments in a Rat Model. Oxid Med Cell Longev 2022:5394303 (2022). PubMed: 35154566
- Liang BJ et al. Intermittent fasting therapy promotes insulin sensitivity by inhibiting NLRP3 inflammasome in rat model. Ann Palliat Med 10:5299-5309 (2021). PubMed: 34107698
- Salem HR & Faried MA Treadmill Exercise Training Ameliorates Functional and Structural Age-Associated Kidney Changes in Male Albino Rats. ScientificWorldJournal 2021:1393372 (2021). PubMed: 34887703
- Almatroodi SA et al. 6-Gingerol, a Bioactive Compound of Ginger Attenuates Renal Damage in Streptozotocin-Induced Diabetic Rats by Regulating the Oxidative Stress and Inflammation. Pharmaceutics 13:N/A (2021). PubMed: 33670981
- Alkhudhayri DA et al. Moringa peregrina leaf extracts produce anti-obesity, hypoglycemic, anti-hyperlipidemic, and hepatoprotective effects on high-fat diet fed rats. Saudi J Biol Sci 28:3333-3342 (2021). PubMed: 34121870