小鼠SCF ELISA试剂盒(ab100740)

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Sandwich ELISA - ab100740 SCF Mouse ELISA Kit
  • Typical standard curve
  • Typical standard curve

Key features and details

  • Sensitivity: 5 pg/ml
  • Range: 2.74 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat

概述

  • 产品名称

    小鼠SCF ELISA试剂盒
    参阅全部 SCF 试剂盒

  • 检测方法

    Colorimetric

  • 样品类型

    Cell culture supernatant, Serum, Plasma

  • 检测类型

    Sandwich (quantitative)

  • 灵敏度

    < 5 pg/ml

  • 范围

    2.74 pg/ml - 2000 pg/ml

  • 回收率

    96 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 98.64 85% - 106%
    Serum 95.86 85% - 105%
    Plasma 94.56 84% - 104%

  • 实验步骤

    Multiple steps standard assay

  • 种属反应性

    与反应: Mouse, Rat

  • 产品概述

    Abcam’s SCF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SCF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse SCF coated on a 96-well plate. Standards and samples are pipetted into the wells and SCF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SCF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SCF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 平台

    Microplate

性能

  • 存放说明

    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    90X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse SCF 2 vials
    Recombinant Mouse SCF Standard (lyophilized) 2 vials
    SCF Microplate (12 x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • 研究领域

  • 功能

    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

  • 疾病相关

    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric

  • 序列相似性

    Belongs to the SCF family.

  • 发展阶段

    Acts in the early stages of hematopoiesis.

  • 翻译后修饰

    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.

  • 细胞定位

    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt

  • 别名

    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SCF_HUMAN
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all

  • 数据库链接

    图片

    • Sandwich ELISA - ab100740 SCF Mouse ELISA Kit
      Sandwich ELISA - ab100740 SCF Mouse ELISA Kit

      SCF measured in various fluids showing quantity (pg) per mL of tested sample

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100740

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100740

    文献 (5)

    发表研究结果有使用 ab100740？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab100740 被引用在 5 文献中.

    • Zhao Q  et al. Endothelial cell-specific expression of serine/threonine kinase 11 modulates dendritic cell differentiation. Nat Commun 13:648 (2022). PubMed: 35115536
    • Li H  et al. Atractylenolide-1 alleviates gastroparesis in diabetic rats by activating the stem cell factor/c-kit signaling pathway. Mol Med Rep 24:N/A (2021). PubMed: 34368880
    • Dergilev KV  et al. Cell Sheet Comprised of Mesenchymal Stromal Cells Overexpressing Stem Cell Factor Promotes Epicardium Activation and Heart Function Improvement in a Rat Model of Myocardium Infarction. Int J Mol Sci 21:N/A (2020). PubMed: 33339427
    • Lu H  et al. Prevention of adverse cardiac remodeling to volume overload in female rats is the result of an estrogen-altered mast cell phenotype. Am J Physiol Heart Circ Physiol 302:H811-7 (2012). Rat . PubMed: 22160000
    • Li J  et al. Stem cell factor is responsible for the rapid response in mature mast cell density in the acutely stressed heart. J Mol Cell Cardiol 53:469-74 (2012). PubMed: 22850284

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question

    Can I use this kit with rat tissues?

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    Answer

    Thank you for your inquiry.

    Unfortunately, we do not have any cross reactivity data for testing rat samples using the mouse stem cell factor ELISA kit (ab100740). Based on online literature though, mouse SCF has ˜93% homology with rat SCF so there is certainly a good chance of getting cross reactivity. However, the only way to know for sure would be to test. If it helps, both the capture and detection antibody in ab100740 are polyclonal goat IgG. While ab100740 is neither designed nor validated with tissue lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. We recommend diluting the samples at least 5-fold with Assay Diluent B (Item E) to minimize any effects of the detergents in the lysis buffer. The samples may need to be diluted further but this would need to be determined by the the end-use empirically.

    Basically, a lysis buffer must meet the following specifications:

    A) has relatively low salt content (700 mM or less)
    B) does not contain sodium azide
    C) does not contain more than 0.1% SDS
    D) does not contain more than 10 mM reducing agents (beta-mercaptoethanol or dithiothreitol).

    This would include any buffers used for immunoprecipitations, including RIPA buffer.

    I hope this information is helpful. Please do not hesitate to contact us again with any further

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    Question

    Precipitate in diluent B and shortage of HRP-streptavidin component.

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    Answer

    Thank you for your call today and for letting us know about the trouble with this kit.
    As we discussed, I'm sending a free of charge kit which should arrive around Tuesday of next week on the order ***. I have requested that the components are checked for precipitation and shortages.
    I apologize again for the problems with this latest kit. Please let me know how this replacement kit works, and let me know if you have any questions or if there is anything else that we can do for you.

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    Question

    Will ab119602 and ab100786 work with urine? Will ab100740 work with rat?

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    Answer

    Thank you for contacting us.

    First, I want to apologize to you for the delay in gathering this information. I hope this did not cause you any inconveniences.

    In regards to using rat tissues in our SCF Mouse ELISA kit, there is no cross reactivity data testing this mouse kit (ab100740) with rat samples so we do not know. However, we do know that the antibody pair is binding somewhere on the extracellular domain of the SCF protein and that mouse and rat share ˜93% homology on the extracellular domain. Therefore there is a good chance that there will be cross reactivity. Nevertheless, the only way to be certain would be to test.



    As to your questions about using urine with ab100786 and ab119602.

    Ab119602 will work with urine future datasheets will include this.

    While ab100786 was not validated with urine samples (only serum, plasma, and cell culture supernatants) these types of kit are very accommodating to many types of samples so I see no reason why urine or any other body fluid for that matter would not be compatible with this kit.



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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