小鼠RANTES ELISA试剂盒(CCL5) (ab100739)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.82 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠RANTES ELISA试剂盒(CCL5)
参阅全部 RANTES 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 1 pg/ml -
范围
0.82 pg/ml - 200 pg/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 114.7 86% - 122% Serum 109.9 98% - 119% Plasma 75.41 68% - 87% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s RANTES Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RANTES in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Mouse RANTES ELISA Kit (ab215537) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse RANTES 2 vials RANTES Microplate (12 x 8 wells) 1 unit Recombinant Mouse RANTES Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. -
组织特异性
T-cell and macrophage specific. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
翻译后修饰
N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. -
细胞定位
Secreted. - Information by UniProt
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别名
- Beta chemokine RANTES
- Beta chemokine RANTES precursor
- C C motif chemokine 5
see all -
数据库链接
- Entrez Gene: 20304 Mouse
- SwissProt: P30882 Mouse
图片
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Typical standard curve
Representative standard curve using ab100739
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Typical standard curveRepresentative standard curve using ab100739
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab100739 被引用在 3 文献中.
- Bayer F et al. Colonization with Altered Schaedler Flora Impacts Leukocyte Adhesion in Mesenteric Ischemia-Reperfusion Injury. Microorganisms 9:N/A (2021). PubMed: 34442681
- Koundouros N et al. Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids. Cell 181:1596-1611.e27 (2020). PubMed: 32559461
- Tokami H et al. RANTES has a potential to play a neuroprotective role in an autocrine/paracrine manner after ischemic stroke. Brain Res 1517:122-32 (2013). Mouse . PubMed: 23602964