小鼠Interferon gamma ELISA试剂盒(without plates) (ab47618)
概述
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产品名称
小鼠Interferon gamma ELISA试剂盒(without plates)
参阅全部 Interferon gamma 试剂盒 -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s IFNγ Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IFNγ in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The murine IFN-gamma ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure murine IFN-gamma in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Murine Interferon gamma Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 15 x 96 tests 5 x 96 tests 10 x 96 tests Chromogen TMB Substrate Solution 6 vials 2 vials 4 vials Detection biotinylated anti-Murine IFN gamma antibody 3 vials 1 vial 2 vials IFN gamma Standard (Lyophilized) Yellow 15 vials 5 vials 10 vials Murine IFN gamma Capture Antibody 3 x 0.25ml 1 x 0.25ml 2 x 0.25ml Streptavidin-HRP 3 vials 1 vial 2 vials -
研究领域
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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别名
- IF 1
- IFG
- IFI
see all -
数据库链接
- Entrez Gene: 15978 Mouse
- SwissProt: P01580 Mouse
- Unigene: 240327 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47618于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Murine Interferon gamma ELISA Set (ab47618)Murine IFN-gamma Standard curve ranging from 31.25 to 1000 pg/mL
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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