小鼠Interferon gamma ELISA试剂盒(ab46081)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Milk, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Interferon gamma ELISA试剂盒
参阅全部 Interferon gamma 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 20 315.4pg/ml 14.5 4.6% B 19 588.6pg/ml 19.1 4.6% 批次间 样品 n Mean SD CV% A 9 128.8pg/ml 7.4 5.7% B 10 546.9pg/ml 25.2 4.6% -
样品类型
Milk, Serum -
检测类型
Sandwich (quantitative) -
灵敏度
< 15 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Mouse interferon gamma ELISA kit (IFN Gamma) is designed for the quantitative measurement of IFN gamma in mouse serum, buffered solutions and cell culture medium.
A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.
This kit will recognize both endogenous and recombinant mouse IFN gamma.
Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml IFN gamma Microplate (12 x 8 well strips) 2 units 1 unit IFN gamma Standard (Lyophilized) Yellow 4 vials 2 vials IFNγ Biotinylated detection antibody 2 x 400µl 1 x 400µl Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究领域
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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别名
- IF 1
- IFG
- IFI
see all -
数据库链接
- Entrez Gene: 15978 Mouse
- SwissProt: P01580 Mouse
- Unigene: 240327 Mouse
相关产品
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SimpleStep ELISA kits
图片
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Typical Standard Curve
Representative Standard Curve using ab46081
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab46081 被引用在 5 文献中.
- Abe S et al. Combination of ultrasound-based mechanical disruption of tumor with immune checkpoint blockade modifies tumor microenvironment and augments systemic antitumor immunity. J Immunother Cancer 10:N/A (2022). PubMed: 35039461
- Khodayari H et al. Gamma-radiated immunosuppressed tumor xenograft mice can be a new ideal model in cancer research. Sci Rep 11:256 (2021). PubMed: 33420261
- Menga A et al. Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages. EMBO Mol Med N/A:e11210 (2020). PubMed: 32885605
- Sousa-Victor P et al. MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage. Nat Metab 1:276-290 (2019). PubMed: 31489403
- Hajmohammadi M et al. Synthesis and characterization of a novel chemically designed (Globo)3-DTPA-KLH antigen. Drug Des Devel Ther 9:217-39 (2015). Sandwich ELISA ; Mouse . PubMed: 25565775