小鼠IL-2酶联免疫斑点法（ELISPOT）Set (ab46623)
Key features and details
- Assay type: Sandwich (qualitative)
- Sample type: Suspension cells
- Reacts with: Mouse
概述
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产品名称
小鼠IL-2酶联免疫斑点法（ELISPOT）Set
参阅全部 IL-2 试剂盒 -
样品类型
Suspension cells -
检测类型
Sandwich (qualitative) -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
The Mouse IL-2 ELISPOT Set is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
This ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principal:
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural murine IL-2.
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经测试应用
适用于: ELISpotmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests Bovine Serum Albumin 1 x 1g 2 x 1g 3 x 1g 4 x 1g Dry Skimmed milk 1 x 1g 2 x 1g 3 x 1g 4 x 1g IL-2 Biotinylated Detection antibody 1 vial 2 vials 3 vials 4 vials Murine IL-2 Capture antibody 1 x 500µl 2 x 500µl 3 x 500µl 4 x 500µl Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 4 x 27ml 3 x 50ml 4 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl 3 x 50µl 4 x 50µl -
研究领域
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相关性
Human interleukin 2 (IL-2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL-2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells. -
细胞定位
Secreted -
别名
- Aldesleukin
- IL 2
- IL-2
see all -
数据库链接
- Entrez Gene: 16183 Mouse
- SwissProt: P04351 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46623于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
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| 说明 |
|---|
|
ELISpot
Use at an assay dependent dilution. |
数据表及文件
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SDS download
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Datasheet download
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