小鼠Eotaxin-1 ELISA试剂盒(ab100680)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 1.02 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Eotaxin-1 ELISA试剂盒
参阅全部 Eotaxin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 1 pg/ml -
范围
1.02 pg/ml - 100 pg/ml -
回收率
94 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 95.25 83% - 104% Serum 94.95 83% - 104% Plasma 92.54 84% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s Eotaxin-1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Eotaxin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Eotaxin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Eotaxin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 80x HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Eotaxin-1 2 vials Eotaxin-1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse Eotaxin-1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
翻译后修饰
O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues. -
细胞定位
Secreted. - Information by UniProt
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别名
- C-C motif chemokine 11
- CCL 11
- CCL11
see all -
数据库链接
- Entrez Gene: 20292 Mouse
- SwissProt: P48298 Mouse
- Unigene: 4686 Mouse
图片
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Typical Standard Curve
Representative standard curve using ab100680
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Typical Standard CurveRepresentative standard curve using ab100680
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab100680 被引用在 3 文献中.
- Li X et al. miR-30a-3p participates in the development of asthma by targeting CCR3. Open Med (Wars) 15:483-491 (2020). PubMed: 33313407
- Ren X et al. FOXM1 promotes allergen-induced goblet cell metaplasia and pulmonary inflammation. Mol Cell Biol 33:371-86 (2013). PubMed: 23149934
- Ci X et al. Different Effects of Farrerol on an OVA-Induced Allergic Asthma and LPS-induced Acute Lung Injury. PLoS One 7:e34634 (2012). PubMed: 22563373