Key features and details
- Sensitivity: 1.3 pg/ml
- Range: 1.25 pg/ml - 80 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
参阅全部 CXCL16 试剂盒
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12%
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 1.3 pg/ml
范围1.25 pg/ml - 80 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 96.71 84% - 103% Serum 94.34 83% - 103% Plasma 93.49 82% - 102%
实验步骤Multiple steps standard assay
Abcam’s CXCL16 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL16 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CXCL16 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL16 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CXCL16 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL16 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CXCL16 2 vials CXCL16 Microplate (12 x 8 wells) 1 unit Recombinant Mouse CXCL16 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
相关性CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. It induces a strong chemotactic response and calcium mobilization. It binds to CXCR6/Bonzo.
细胞定位Cell Membrane and Secreted
ab100677 被引用在 2 文献中.
- Kuriyama H et al. Immunotherapy with 4-1BBL-Expressing iPS Cell-Derived Myeloid Lines Amplifies Antigen-Specific T Cell Infiltration in Advanced Melanoma. Int J Mol Sci 22:N/A (2021). PubMed: 33669419
- Wu J et al. Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke. Aging Dis 12:786-800 (2021). PubMed: 34094642