小鼠CXCL1 ELISA试剂盒 (GRO alpha) (ab216951)
Key features and details
- Sensitivity: 0.66 pg/ml
- Range: 5.47 pg/ml - 350 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠CXCL1 ELISA试剂盒 (GRO alpha)
参阅全部 CXCL1/GRO alpha 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Mouse serum 8 6.8% 批次间 样品 n Mean SD CV% Mouse serum 3 10.1% -
样品类型
Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.66 pg/ml -
范围
5.47 pg/ml - 350 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 91 88% - 94% Cell culture extracts 95 95% - 96% Cell culture media 110 109% - 110% Hep Plasma 79 72% - 90% EDTA Plasma 94 89% - 98% Cit plasma 94 92% - 97% -
检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse
不与反应: Cow -
产品概述
Mouse CXCL1 ELISA Kit (GRO alpha) (ab216951) sandwich ELISA designed for the quantitative measurement of CXCL1 (GRO alpha) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It quantitates Mouse CXCL1 with 0.66 pg/ml sensitivity. The recombinant antibodies used in this kit guarantee specificity and consistency of results.
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说明
CXCL1 is a member of the CXC family of chemokines. Chemokines play roles in normal and pathological processes including allergic responses, angiogenesis, inflammation, tumor growth and metastasis. Mouse CXCL2 and CXCL3 share 67% and 60% sequence homology with mouse CXCL1, respectively. Additionally, Rat CXCL1 is 89% homologous with mouse CXCL1.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse CXCL1 Capture Antibody 1 x 600µl 10X Mouse CXCL1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 2 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI2 2 x 6ml Mouse CXCL1 Lyophilized Recombinant Protein (ab9906) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
数据库链接
- Entrez Gene: 14825 Mouse
- SwissProt: P12850 Mouse
相关产品
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Alternative Versions
图片
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Standard curve comparison between mouse CXCL1 SimpleStep ELISA® kit (GRO alpha) and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold sensitivity.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 386.84 pg/mL in neat serum, 399.45 pg/mL in neat plasma (citrate), 364.34 pg/mL in neat plasma (EDTA), and 505.80 pg/mL in neat plasma (heparin).
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The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 48.56 pg/mL in neat serum, 24.34 pg/mL in neat plasma (citrate), 23.67 pg/mL in neat plasma (EDTA), and 176.56 pg/mL in neat plasma (heparin).
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The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: NIH 3T3 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 472.88 pg/mL in neat mouse NIH 3T3 cell culture supernatant.
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The concentrations of CXCL1 (GRO alpha) were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 89.05 pg/mL in mouse NIH 3T3 cell extract sample.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab216951 被引用在 6 文献中.
- Jia P et al. LncRNA IRAR regulates chemokines production in tubular epithelial cells thus promoting kidney ischemia-reperfusion injury. Cell Death Dis 13:562 (2022). PubMed: 35732633
- Klapproth E et al. Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction. Nat Commun 13:7648 (2022). PubMed: 36496449
- Xu Y et al. L-Theanine Alleviates IMQ-Induced Psoriasis Like Skin Inflammation by Downregulating the Production of IL-23 and Chemokines. Front Pharmacol 12:719842 (2021). PubMed: 34381369
- Winkler MJ et al. Functional investigation of the coronary artery disease gene SVEP1. Basic Res Cardiol 115:67 (2020). PubMed: 33185739
- Fang C et al. Berberine ameliorates neonatal necrotizing enterocolitis by activating the phosphoinositide 3-kinase/protein kinase B signaling pathway. Exp Ther Med 15:3530-3536 (2018). PubMed: 29545879
- Yan B et al. HMGB1-TLR4-IL23-IL17A axis promotes paraquat-induced acute lung injury by mediating neutrophil infiltration in mice. Sci Rep 7:597 (2017). PubMed: 28377603