mCherry ELISA试剂盒(ab221829)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.47 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
概述
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产品名称
mCherry ELISA试剂盒
参阅全部 mCherry 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Media 8 2.9% 批次间 样品 n Mean SD CV% Media 3 3.7% -
样品类型
Cell culture supernatant, Cell culture extracts, Cell culture media -
检测类型
Sandwich (quantitative) -
灵敏度
0.47 pg/ml -
范围
6.25 pg/ml - 400 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 111 110% - 113% Cell culture extracts 100 97% - 102% Cell culture media 112 111% - 112% -
检测时间
1h 30m -
实验步骤
One step assay -
产品概述
mCherry ELISA Kit (ab221829) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of mCherry protein in cell culture extracts, cell culture media, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate mCherry with 0.47 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
This kit recognizes mCherry fluorescent protein from the organism Discosoma sp. mCherry is a general-purpose, red monomer, fluorescent protein with superior photostability. The mCherry fluorescent protein is a variant of the Discosoma red (DsRed) protein and is excited at wavelengths longer than 600 nm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X mCherry Capture Antibody 1 x 600µl 10X mCherry Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BC 1 x 6ml mCherry Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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相关性
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
相关产品
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Alternative Versions
图片
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of mCherry were measured in duplicates, interpolated from the mCherry standard curves and corrected for sample dilution. Undiluted samples are as follows: RPMI culture media with 10% FBS (100%), and HUVEC supernatant (100%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean mCherry concentration was determined to be 204.81 pg/mL in spiked RPMI culture media with 10% FBS (100%) and 212.94 pg/mL in spiked HUVEC supernatant (100%). HUVEC cells were cultured in RPMI media with 10% FBS and supernatant collected.
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The concentrations of mCherry were measured in duplicate and interpolated from the mCherry standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean mCherry concentration was determined to be 182.08 pg/mL in spiked HEK 293T cell extract and 198.36 pg/mL in spiked 143B cell extract.
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All proteins were loaded at 400 pg/mL. The average background O.D. is represented by the dashed line. Signal from YFP, GFP, and RFP loaded at 400 pg/mL was equivalent to background.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab221829 被引用在 2 文献中.
- Chen Z et al. Transgenic zebrafish modeling low-molecular-weight proteinuria and lysosomal storage diseases. Kidney Int 97:1150-1163 (2020). PubMed: 32061435
- Reid CA et al. Development of an inducible anti-VEGF rAAV gene therapy strategy for the treatment of wet AMD. Sci Rep 8:11763 (2018). PubMed: 30082848